Human Predicted to work with:
Mouse, Rat, Cow, Orangutan
Recombinant fragment: PDSVPVDSFP PAPSMAVVAV LESTLSCEAC KNGMPTISRL LQMPLFSDVL LTTSEKPQFK IPTKLKEALR IAKECIEKRL IEEQKQIHQH RRLTRAQSHH, corresponding to amino acids 351-450 of Human PXK (AAH14479) with a proprietory tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 65 kDa.
Use at an assay dependent dilution.
FunctionBinds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.
Tissue specificityWidely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated.
Sequence similaritiesBelongs to the protein kinase superfamily. Contains 1 protein kinase domain. Contains 1 PX (phox homology) domain. Contains 1 WH2 domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
Cellular localizationCytoplasm. Cell membrane. Also associates with the plasma membrane. Isoform 3 is present throughout the cell.