Overview

  • Product nameAnti-PXK antibody
    See all PXK primary antibodies
  • Description
    Mouse monoclonal to PXK
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Recombinant fragment: PDSVPVDSFP PAPSMAVVAV LESTLSCEAC KNGMPTISRL LQMPLFSDVL LTTSEKPQFK IPTKLKEALR IAKECIEKRL IEEQKQIHQH RRLTRAQSHH, corresponding to amino acids 351-450 of Human PXK (AAH14479) with a proprietory tag.

  • Positive control
    • PXK transfected 293T cell lysate. Recombinant tagged human PXK fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionBinds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.
  • Tissue specificityWidely expressed in all tissues examined except in heart. Isoform 1 is expressed in high levels in the brain, skeletal muscle, spleen and testis. Isoform 7 expression has yet to be demonstrated.
  • Sequence similaritiesBelongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
    Contains 1 PX (phox homology) domain.
    Contains 1 WH2 domain.
  • DomainThe protein kinase domain is predicted to be catalytically inactive.
  • Cellular localizationCytoplasm. Cell membrane. Also associates with the plasma membrane. Isoform 3 is present throughout the cell.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ20335 antibody
    • K-ATPase antibody
    • Modulator of Na antibody
    • Modulator of Na K ATPase antibody
    • Modulator of Na K ATPase long form antibody
    • MONaKA antibody
    • PX domain containing protein kinase like protein antibody
    • PX domain containing serine/threonine kinase antibody
    • PX domain-containing protein kinase-like protein antibody
    • PX ser/thr kinase v2 antibody
    • PX serine/threonine kinase antibody
    • Pxk antibody
    • PXK_HUMAN antibody
    see all

Anti-PXK antibody images

  • Anti-PXK antibody (ab77652) at 1 µg/ml + Recombinant tagged human PXK fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 65 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PXK antibody (ab77652) at 1 µg/ml

    Lane 1 : PXK transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 65 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

References for Anti-PXK antibody (ab77652)

ab77652 has not yet been referenced specifically in any publications.

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