Overview

  • Product nameAnti-PXR antibody
    See all PXR primary antibodies
  • Description
    Mouse polyclonal to PXR
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-434 of Human PXR (NP_003880.3).

  • Positive control
    • Rat liver tissue lysate; PXR transfected 293T cell line lysate; HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab118336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionNuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
  • Tissue specificityExpressed in liver, colon and small intestine.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BXR antibody
    • Nr1i2 antibody
    • NR1I2_HUMAN antibody
    • Nuclear receptor subfamily 1 group I member 2 antibody
    • ONR 1 antibody
    • ONR1 antibody
    • Orphan nuclear receptor PAR 1 antibody
    • Orphan nuclear receptor PAR1 antibody
    • Orphan nuclear receptor PXR antibody
    • OTTHUMP00000215173 antibody
    • OTTHUMP00000215174 antibody
    • OTTHUMP00000215175 antibody
    • PAR 1 antibody
    • PAR 2 antibody
    • PAR antibody
    • PAR q antibody
    • PAR1 antibody
    • PAR2 antibody
    • PARq antibody
    • pregnane X nuclear receptor variant 2 antibody
    • Pregnane X receptor antibody
    • PRR antibody
    • PXR antibody
    • SAR antibody
    • Steroid and xenobiotic receptor antibody
    • SXR antibody
    see all

Anti-PXR antibody images

  • Anti-PXR antibody (ab118336) at 1 µg/ml + Rat liver tissue lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 50 kDa
  • All lanes : Anti-PXR antibody (ab118336) at 1 µg/ml

    Lane 1 : PXR transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 50 kDa
  • ab118336 at 10 µg/ml dilution staining PXR in HepG2 cells by Immunofluorescence.

References for Anti-PXR antibody (ab118336)

ab118336 has not yet been referenced specifically in any publications.

Product Wall


I am happy to confirm you that this antibody will work using formaldehyde fixed cells. I can confirm that the image provided on the datasheet was obtained using 4% paraformaldehyde solution as fixative.

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I am sorry that the antibody, ab118336 did not perform as stated on the datasheet and I would be happy to process a refund for the cost of the antibody. To be able to complete the refund, would you please be able to p...

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Thank you for contacting Abcam.

I have attached the protocol that was used to test ab118336 in IF, the protocol is similar to yours but there are a few differences that would be worth trying out.

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