Anti-PXR antibody (ab118336)
Key features and details
- Mouse polyclonal to PXR
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PXR antibody
See all PXR primary antibodies -
Description
Mouse polyclonal to PXR -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-434 of Human PXR (NP_003880.3).
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Positive control
- Rat liver tissue lysate; PXR transfected 293T cell line lysate; HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes. -
Tissue specificity
Expressed in liver, colon and small intestine. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8856 Human
- Entrez Gene: 84385 Rat
- Omim: 603065 Human
- SwissProt: O75469 Human
- SwissProt: Q9R1A7 Rat
- Unigene: 7303 Human
- Unigene: 207164 Rat
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Alternative names
- BXR antibody
- Nr1i2 antibody
- NR1I2_HUMAN antibody
see all
Images
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Anti-PXR antibody (ab118336) at 1 µg/ml + Rat liver tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa -
All lanes : Anti-PXR antibody (ab118336) at 1 µg/ml
Lane 1 : PXR transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 50 kDa -
ab118336 at 10 µg/ml dilution staining PXR in HepG2 cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab118336 has been referenced in 3 publications.
- Ortiz-Flores M et al. PXR is a target of (-)-epicatechin in skeletal muscle. Heliyon 6:e05357 (2020). PubMed: 33163657
- Jang M et al. Study of melatonin-mediated effects on various hepatic inflammatory responses stimulated by IL-6 in a new HepG2-on-a-chip platform. Biomed Microdevices 20:54 (2018). PubMed: 29946898
- Jing W et al. Berberine Upregulates P-Glycoprotein in Human Caco-2 Cells and in an Experimental Model of Colitis in the Rat via Activation of Nrf2-Dependent Mechanisms. J Pharmacol Exp Ther 366:332-340 (2018). PubMed: 29891588