Overview

  • Product name
  • Description
    Rabbit polyclonal to PXR
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (KKEMIMSDEA VEERRALIKR KKSERTGTQP LGVQGLTEEQ RMMIRELMDA) of Human PXR (NP_003880).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
  • Tissue specificity
    Expressed in liver, colon and small intestine.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BXR antibody
    • Nr1i2 antibody
    • NR1I2_HUMAN antibody
    • Nuclear receptor subfamily 1 group I member 2 antibody
    • ONR 1 antibody
    • ONR1 antibody
    • Orphan nuclear receptor PAR 1 antibody
    • Orphan nuclear receptor PAR1 antibody
    • Orphan nuclear receptor PXR antibody
    • OTTHUMP00000215173 antibody
    • OTTHUMP00000215174 antibody
    • OTTHUMP00000215175 antibody
    • PAR 1 antibody
    • PAR 2 antibody
    • PAR antibody
    • PAR q antibody
    • PAR1 antibody
    • PAR2 antibody
    • PARq antibody
    • pregnane X nuclear receptor variant 2 antibody
    • Pregnane X receptor antibody
    • PRR antibody
    • PXR antibody
    • SAR antibody
    • Steroid and xenobiotic receptor antibody
    • SXR antibody
    see all

Images

  • Anti-PXR antibody (ab85451) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling PXR with ab85451 at 5.0µg/ml.

References

This product has been referenced in:
  • Kong Q  et al. Co-expression of pregnane X receptor and ATP-binding cassette sub-family B member 1 in peripheral blood: A prospective indicator for drug resistance prediction in non-small cell lung cancer. Oncol Lett 11:3033-3039 (2016). IHC . Read more (PubMed: 27123059) »
  • Dou W  et al. Plant flavonol isorhamnetin attenuates chemically induced inflammatory bowel disease via a PXR-dependent pathway. J Nutr Biochem 25:923-33 (2014). Read more (PubMed: 24913217) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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