Overview

  • Product nameAnti-PYCR1 antibody
    See all PYCR1 primary antibodies
  • Description
    Rabbit polyclonal to PYCR1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide from a region within internal sequence amino acids 216-265 (MLLHSEQHPG QLKDNVSSPG GATIHALHVL ESGGFRSLLI NAVEASCIRT) of Human PYCR1 (NP_722546)

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevancePYCR1 is an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion.
  • Cellular localizationMitochondrial
  • Database links
  • Alternative names
    • P5C antibody
    • P5C reductase 1 antibody
    • P5C reductase antibody
    • P5CR 1 antibody
    • P5CR antibody
    • PIG45 antibody
    • PP222 antibody
    • Proliferation inducing protein 45 antibody
    • PYCR 1 antibody
    • PYCR antibody
    • Pyrroline 5 carboxylate reductase 1 antibody
    • Pyrroline 5 carboxylate reductase 1 mitochondrial antibody
    see all

Anti-PYCR1 antibody images



  • Predicted band size : 33 kDa
    Western blot analysis of 293T cell lysate labeling PYCR1 with ab94780 at 1 µg/ml.
  • All lanes : Anti-PYCR1 antibody (ab94780) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate at 18 µg
    Lane 2 : HeLa whole cell lysate at 36 µg
    Lane 3 : RPE-1 whole cell lysate at 18 µg
    Lane 4 : RPE-1 whole cell lysate at 36 µg

    Secondary
    LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 7 minutes

    This image is courtesy of an anonymous abreview.

    Blocking:10% Milk

    See Abreview

  • Anti-PYCR1 antibody (ab94780) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Gel concentration: 12%

References for Anti-PYCR1 antibody (ab94780)

ab94780 has not yet been referenced specifically in any publications.

Product Wall

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa, RPE-1)
Specification HeLa, RPE-1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 2°C
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Submitted May 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"