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Rabbit polyclonal to PYCR1
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide from a region within internal sequence amino acids 216-265 (MLLHSEQHPG QLKDNVSSPG GATIHALHVL ESGGFRSLLI NAVEASCIRT) of Human PYCR1 (NP_722546)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
PYCR1 is an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion.
P5C reductase 1 antibody
P5C reductase antibody
Western blot - Anti-PYCR1 antibody (ab94780)
Predicted band size : 33 kDa Western blot analysis of 293T cell lysate labeling PYCR1 with ab94780 at 1 µg/ml.
Western blot - Anti-PYCR1 antibody (ab94780) This image is courtesy of an anonymous abreview.
All lanes :
Anti-PYCR1 antibody (ab94780) at 1/1000 dilution
Lane 1 :
HeLa whole cell lysate at 18 µg
Lane 2 :
HeLa whole cell lysate at 36 µg
Lane 3 :
RPE-1 whole cell lysate at 18 µg
Lane 4 :
RPE-1 whole cell lysate at 36 µg
LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution
Performed under reducing conditions.
Predicted band size :
Observed band size :
34 kDa (
why is the actual band size different from the predicted?
Exposure time :
This image is courtesy of an anonymous abreview. Blocking:10% Milk
Western blot - PYCR1 antibody (ab94780)
Anti-PYCR1 antibody (ab94780) at 1 µg/ml + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 33 kDa Gel concentration: 12%
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"