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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Relevance PYCR1 is an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. It forms a homopolymer and localizes to the mitochondrion.
Cellular localization Mitochondrial
P5C reductase 1 antibody
P5C reductase antibody
Anti-PYCR1 antibody images
Western blot - Anti-PYCR1 antibody (ab94780)
Predicted band size : 33 kDa Western blot analysis of 293T cell lysate labeling PYCR1 with ab94780 at 1 µg/ml.
Western blot - Anti-PYCR1 antibody (ab94780) This image is courtesy of an anonymous abreview.
All lanes : Anti-PYCR1 antibody (ab94780) at 1/1000 dilution Lane 1 : HeLa whole cell lysate at 18 µg Lane 2 : HeLa whole cell lysate at 36 µg Lane 3 : RPE-1 whole cell lysate at 18 µg Lane 4 : RPE-1 whole cell lysate at 36 µg Secondary LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution Performed under reducing conditions. Predicted band size : 33 kDa Observed band size : 34 kDa ( why is the actual band size different from the predicted?) Exposure time : 7 minutes This image is courtesy of an anonymous abreview. Blocking:10% Milk
Western blot - PYCR1 antibody (ab94780)
Anti-PYCR1 antibody (ab94780) at 1 µg/ml + HepG2 cell lysate at 10 µg Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution Predicted band size : 33 kDa Gel concentration: 12%
References for Anti-PYCR1 antibody (ab94780)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"