Overview

  • Product nameAnti-PYCR2 antibody
    See all PYCR2 primary antibodies
  • Description
    Rabbit polyclonal to PYCR2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-320 of Human PYCR2 (NP_037460.2).

  • Positive control
    • HeLa cell lysate; PYCR2 transfected 293T cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab103535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.

Target

  • FunctionHousekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH.
  • Tissue specificityDetected in erythrocytes (at protein level).
  • PathwayAmino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
  • Sequence similaritiesBelongs to the pyrroline-5-carboxylate reductase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1810018M05Rik antibody
    • FLJ54750 antibody
    • Leftb antibody
    • OTTHUMP00000035614 antibody
    • P5C reductase 2 antibody
    • P5CR 2 antibody
    • P5CR2 antibody
    • P5CR2_HUMAN antibody
    • PYCR 2 antibody
    • PYCR2 antibody
    • Pyrroline 5 carboxylate reductase 2 antibody
    • Pyrroline 5 carboxylate reductase family member 2 antibody
    • Pyrroline 5 carboxylate reductase isoform antibody
    • Pyrroline-5-carboxylate reductase 2 antibody
    • RP4-559A3.4 antibody
    see all

Anti-PYCR2 antibody images

  • All lanes : Anti-PYCR2 antibody (ab103535) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate- 18µg
    Lane 2 : HeLa whole cell lysate- 36µg
    Lane 3 : RPE-1 whole cell lysate- 18µg
    Lane 4 : RPE-1 whole cell lysate- 36µg

    Secondary
    LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 7 minutes

    This image is courtesy of an anonymous abreview.

    Blocking: 10% Milk

    See Abreview

  • Anti-PYCR2 antibody (ab103535) at 1/500 dilution + HeLa cell lysate at 50 µg

    Predicted band size : 34 kDa
  • All lanes : Anti-PYCR2 antibody (ab103535) at 1/500 dilution

    Lane 1 : PYCR2 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa

References for Anti-PYCR2 antibody (ab103535)

ab103535 has not yet been referenced specifically in any publications.

Product Wall

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 38 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa, RPE-1)
Specification HeLa, RPE-1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted May 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"