- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-PYCR2 antibody
See all PYCR2 primary antibodies -
DescriptionRabbit polyclonal to PYCR2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey, Orangutan
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Immunogen
Recombinant fragment corresponding to Human PYCR2 aa 282-319.
Sequence:LKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKK
Database link: Q96C36 -
Positive control
- Human rectum tissue; RT4 and U251MG sp cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab185578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/250. Predicted molecular weight: 34 kDa. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionHousekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH.
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Tissue specificityDetected in erythrocytes (at protein level).
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PathwayAmino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
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Sequence similaritiesBelongs to the pyrroline-5-carboxylate reductase family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 29920 Human
- Entrez Gene: 100172597 Orangutan
- SwissProt: Q4R6W7 Cynomolgus monkey
- SwissProt: Q96C36 Human
- SwissProt: Q5RAQ3 Orangutan
- Unigene: 654718 Human
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Alternative names
- 1810018M05Rik antibody
- FLJ54750 antibody
- Leftb antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR2 antibody (ab185578)Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling PYCR2 with ab185578 at 1/500 dilution.
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Western blot - Anti-PYCR2 antibody (ab185578)All lanes : Anti-PYCR2 antibody (ab185578) at 1/100 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251MG sp cell lysate
Predicted band size: 34 kDa
References
ab185578 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"