Overview

  • Product name
  • Description
    Rabbit polyclonal to PYCR2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus monkey, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human PYCR2 aa 282-319.
    Sequence:

    LKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKK


    Database link: Q96C36

  • Positive control
    • Human rectum tissue; RT4 and U251MG sp cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab185578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 34 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH.
  • Tissue specificity
    Detected in erythrocytes (at protein level).
  • Pathway
    Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
  • Sequence similarities
    Belongs to the pyrroline-5-carboxylate reductase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1810018M05Rik antibody
    • FLJ54750 antibody
    • Leftb antibody
    • OTTHUMP00000035614 antibody
    • P5C reductase 2 antibody
    • P5CR 2 antibody
    • P5CR2 antibody
    • P5CR2_HUMAN antibody
    • PYCR 2 antibody
    • PYCR2 antibody
    • Pyrroline 5 carboxylate reductase 2 antibody
    • Pyrroline 5 carboxylate reductase family member 2 antibody
    • Pyrroline 5 carboxylate reductase isoform antibody
    • Pyrroline-5-carboxylate reductase 2 antibody
    • RP4-559A3.4 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human rectum tissue labeling PYCR2 with ab185578 at 1/500 dilution.

  • All lanes : Anti-PYCR2 antibody (ab185578) at 1/100 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U251MG sp cell lysate


    Predicted band size : 34 kDa

References

ab185578 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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