Overview

  • Product nameAnti-PYGO1 antibody
    See all PYGO1 primary antibodies
  • Description
    Rabbit polyclonal to PYGO1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 369-419 of Human PYGO1 (NP_056432.1).

  • Positive control
    • Whole cell lysate from HeLa and 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

Anti-PYGO1 antibody images

  • All lanes : Anti-PYGO1 antibody (ab95072) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 45 kDa


    Exposure time : 3 minutes
  • Detection of PYGO1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab95072 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-PYGO1 antibody (ab95072)

ab95072 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"