Anti-PYGO1 antibody - C-terminal (ab170607)

Overview

  • Product name
    Anti-PYGO1 antibody - C-terminal
    See all PYGO1 primary antibodies
  • Description
    Rabbit polyclonal to PYGO1 - C-terminal
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PYGO1 aa 389-417 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9Y3Y4

  • Positive control
    • ZR-75-1 cells; ZR-75-1 cell lysate; Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent analysis of ZR-75-1 cells labeling PYGO1 with ab170607 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). Actin filaments were labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nucleus (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling PYGO1 with ab170607 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining

  • Anti-PYGO1 antibody - C-terminal (ab170607) at 1/100 dilution + ZR-75-1 cell lysate at 35 µg

    Predicted band size : 45 kDa

References

ab170607 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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