Anti-PYGO2 antibody (ab155262)
Key features and details
- Rabbit polyclonal to PYGO2
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PYGO2 antibody
See all PYGO2 primary antibodies -
Description
Rabbit polyclonal to PYGO2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PYGO2 aa 1-368.
Database link: Q9BRQ0 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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IP |
Use at an assay dependent concentration.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IP
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). |
Target
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Function
Involved in signal transduction through the Wnt pathway. -
Sequence similarities
Contains 1 PHD-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 90780 Human
- Entrez Gene: 68911 Mouse
- Entrez Gene: 295251 Rat
- Omim: 606903 Human
- SwissProt: Q9BRQ0 Human
- Unigene: 533597 Human
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Alternative names
- 1190004M21Rik antibody
- FLJ33226 antibody
- PP7910 antibody
see all
Images
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Anti-PYGO2 antibody (ab155262) at 1/1000 dilution + mouse NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 41 kDa
10% SDS PAGE -
Immunoprecipitation of PYGO2 from SW480 whole cell lysate (5 X 107 cells) using 1 ug of ab155262. The precipitated PYGO2 was detected using ab155262 diluted at 1:15000.
Lane 1: IP-IgG
Lane 2: IP-PYGO2
All lanes : Anti-PYGO2 antibody (ab155262) at 1/15000 dilution
Lane 1 : IP negative control - SW480 whole cell lysate, corresponding to 5*107 cells, incubated with a IgG.
Lane 2 : SW480 whole cell lysate, corresponding to 5*107 cells, immunoprecipitated with 1 µg of ab155262 -
4% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells labeling PYGO2 using ab155262 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Paraffin-embedded rat intestine tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Paraffin-embedded mouse stomach tissue labeleing PYGO2 using ab155262 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocarcinoma tissue, labeling PYGO2 using ab155262 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155262 has not yet been referenced specifically in any publications.