Overview

  • Product name
  • Description
    Rabbit polyclonal to Pygopus 2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-368 of Human Pygopus 2 (Uniprot ID#Q9BRQ0)

  • Positive control
    • Human SW480 cell lysate; Mouse NIH 3T3 cell lysate; Human lung adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

Images

  • Immunoprecipitation of Pygopus 2 from SW480 whole cell lysate (5 X 107 cells) using 1 ug of ab155262. The precipitated Pygopus 2 was detected using ab155262 diluted at 1:15000.
    Lane 1: IP-IgG
    Lane 2: IP-Pygopus 2
  • Anti-Pygopus 2 antibody (ab155262) at 1/1000 dilution + mouse NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 41 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocarcinoma tissue, labeling Pygopus 2 using ab155262 at a 1/500 dilution.

References

ab155262 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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