Overview

  • Product nameAnti-Pygopus 2 antibody
    See all Pygopus 2 primary antibodies
  • Description
    Rabbit polyclonal to Pygopus 2
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 of Human Pygopus 2 (NP_612157.1).

  • Positive control
    • HeLa, 293T and Mouse NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-Pygopus 2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pygopus 2 with ab99274 at 1/200 (1µg/mg). Detection: DAB.
  • All lanes : Anti-Pygopus 2 antibody (ab99274) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 30 seconds
  • ab99274, at 0.4 µg/ml, detecting Pygopus 2 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
    For immunoprecipitation, ab99274 was used at 3 µg/mg lysate (1 mg of lysate was used for IP and 20% of IP was loaded).
    Lane 1; IP using ab99274
    Lane 2; IP using a rabbit anti-Pygopus 2 antibody which recognizes a downstream epitope.
    Lane 3; IP using control IgG.

References for Anti-Pygopus 2 antibody (ab99274)

ab99274 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99274.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"