Overview

  • Product name
  • Description
    Rabbit polyclonal to Pygopus 2
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 of Human Pygopus 2 (NP_612157.1).

  • Positive control
    • HeLa, 293T and Mouse NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Pygopus 2 with ab99274 at 1/200 (1µg/mg). Detection: DAB.
  • All lanes : Anti-Pygopus 2 antibody (ab99274) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 30 seconds
  • ab99274, at 0.4 µg/ml, detecting Pygopus 2 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
    For immunoprecipitation, ab99274 was used at 3 µg/mg lysate (1 mg of lysate was used for IP and 20% of IP was loaded).
    Lane 1; IP using ab99274
    Lane 2; IP using a rabbit anti-Pygopus 2 antibody which recognizes a downstream epitope.
    Lane 3; IP using control IgG.

References

ab99274 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab99274.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up