Overview

  • Product nameAnti-Pygopus 2 antibody [EPR2024(2)]
    See all Pygopus 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2024(2)] to Pygopus 2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Pygopus 2.

  • Positive control
    • MCF7, HeLa, Human ovary cancer, and NCCIT cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    Anti-Pygopus 2 antibody [EPR2024(2)] images

    • All lanes : Anti-Pygopus 2 antibody [EPR2024(2)] (ab109001) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human ovary cancer lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa

    References for Anti-Pygopus 2 antibody [EPR2024(2)] (ab109001)

    This product has been referenced in:
    • Li B  et al. Repurposing the FDA-approved pinworm drug pyrvinium as a novel chemotherapeutic agent for intestinal polyposis. PLoS One 9:e101969 (2014). WB ; Human . Read more (PubMed: 25003333) »
    • Liu Y  et al. Abnormal expression of Pygopus 2 correlates with a malignant phenotype in human lung cancer. BMC Cancer 13:346 (2013). WB, IHC . Read more (PubMed: 23865714) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (primary myoblast)
    Specification primary myoblast
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative Paraformaldehyde
    Username

    Fabien LE GRAND

    Verified customer

    Submitted Jun 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"