Recombinant
RabMAb

Anti-Pygopus 2 antibody [EPR2024(2)] (ab109001)

Overview

  • Product name
    Anti-Pygopus 2 antibody [EPR2024(2)]
    See all Pygopus 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2024(2)] to Pygopus 2
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Pygopus 2.

  • Positive control
    • MCF7, HeLa, Human ovary cancer, and NCCIT cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    Images

    • All lanes : Anti-Pygopus 2 antibody [EPR2024(2)] (ab109001) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human ovary cancer lysate
      Lane 4 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 41 kDa

    References

    This product has been referenced in:
    • Zhang S  et al. Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression. Oncotarget 6:11074-86 (2015). WB, IHC . Read more (PubMed: 25871475) »
    • Li B  et al. Repurposing the FDA-approved pinworm drug pyrvinium as a novel chemotherapeutic agent for intestinal polyposis. PLoS One 9:e101969 (2014). WB ; Human . Read more (PubMed: 25003333) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (primary myoblast)
    Specification
    primary myoblast
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Fabien Le Grand

    Verified customer

    Submitted Jun 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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