Overview

  • Product nameAnti-PYHIN1 antibody
  • Description
    Rabbit polyclonal to PYHIN1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, African Green Monkey
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (ANNYKKIVLL KGLEVINDYH FRIVKSLLSN DLKLNPKMKE EYDKIQIADL) of Human PYHIN1 (NP_689714).

  • Positive control
    • MCF7 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMajor mediator of the tumor suppressor activity of IFN in breast cancer cells. Promotes ubiquitination and subsequent degradation of MDM2, which leads to p53/TP53 stabilization. Promotes ubiquitination and subsequent degradation of HDAC1, which in turn enhances maspin expression, and impairs invasive activity of cancer cells.
  • Tissue specificityExpressed in spleen, lymph node and peripheral blood leukocytes, and at lower levels in thymus, bone marrow and fetal liver. Down-regulated in breast tumors.
  • Sequence similaritiesBelongs to the HIN-200 family.
    Contains 1 DAPIN domain.
    Contains 1 HIN-200 domain.
  • DomainThe HIN-200 domain mediates interaction with MDM2.
  • Cellular localizationNucleus > nucleoplasm and Nucleus. Nucleus speckle.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFIX_HUMAN antibody
    • Interferon-inducible protein X antibody
    • Pyhin1 antibody
    • Pyrin and HIN domain-containing protein 1 antibody

Anti-PYHIN1 antibody images

  • Anti-PYHIN1 antibody (ab106144) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Predicted band size : 55 kDa

References for Anti-PYHIN1 antibody (ab106144)

ab106144 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (RAW 264.7 cells)
Gel Running Conditions Reduced Denaturing (12% gel (Acrylamide))
Loading amount 20 µg
Treatment Interferon bete (2h, 6h, 12h, 24h)
Specification RAW 264.7 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Mar 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"