The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
Is unsuitable for Flow Cyt,ICC or IHC.
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain.
Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
Calcium regulated non receptor proline rich tyrosine kinase antibody
Calcium-dependent tyrosine kinase antibody
Cell adhesion kinase beta antibody
EC 126.96.36.199 antibody
FADK 2 antibody
Focal adhesion kinase 2 antibody
Proline-rich tyrosine kinase 2 antibody
Protein kinase B antibody
Protein Tyrosine Kinase 2 Beta antibody
Protein-tyrosine kinase 2-beta antibody
PTK2B protein tyrosine kinase 2 beta antibody
Related adhesion focal tyrosine kinase antibody
Reprobe control - Anti-PYK2 antibody [E354] (ab32448)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg) Lane 2: PYK2 knockout HAP1 whole cell lysate (20 µg) Lane 3: Jurkat whole cell lysate (20 µg) Lane 4: Hu brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32448 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32448 was shown to specifically react with PYK2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when PYK2 knockout samples were used. Wild-type and PYK2 knockout samples were subjected to SDS-PAGE. Ab32448 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-PYK2 antibody [E354] (ab32448)