• Product nameAnti-PYK2 antibody [EP206Y]
    See all PYK2 primary antibodies
  • Description
    Rabbit monoclonal [EP206Y] to PYK2
  • Tested applicationsSuitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminal of human PYK2.

  • Positive control
    • human brain, rat brain lysates and HeLa cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab81266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
IHC-P 1/100 - 1/250.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionInvolved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
    • Tissue specificityMost abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • Post-translational
      Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
    • Cellular localizationCytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Information by UniProt
    • Database links
    • Alternative names
      • CADTK antibody
      • CAK-beta antibody
      • CAKB antibody
      • CAKbeta antibody
      • Calcium regulated non receptor proline rich tyrosine kinase antibody
      • Calcium-dependent tyrosine kinase antibody
      • Cell adhesion kinase beta antibody
      • E430023O05Rik antibody
      • EC antibody
      • FADK 2 antibody
      • FADK2 antibody
      • FAK2 antibody
      • FAK2_HUMAN antibody
      • Focal adhesion kinase 2 antibody
      • MGC124628 antibody
      • PKB antibody
      • Proline-rich tyrosine kinase 2 antibody
      • Protein kinase B antibody
      • Protein Tyrosine Kinase 2 Beta antibody
      • Protein-tyrosine kinase 2-beta antibody
      • PTK antibody
      • PTK2B antibody
      • PTK2B protein tyrosine kinase 2 beta antibody
      • PYK2 antibody
      • RAFTK antibody
      • RAFTK2 antibody
      • Related adhesion focal tyrosine kinase antibody
      see all

    Anti-PYK2 antibody [EP206Y] images

    • All lanes : Anti-PYK2 antibody [EP206Y] (ab81266) at 1/2000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 116 kDa
      Observed band size : 116 kDa
    • Immunohistochemical analysis of paraffin-embedded human brain using ab81266 at 1/100 dilution.

    References for Anti-PYK2 antibody [EP206Y] (ab81266)

    ab81266 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab81266.
    Please use the links above to contact us or submit feedback about this product.