Overview

  • Product nameAnti-PYK2 antibody [YE353]
    See all PYK2 primary antibodies
  • Description
    Rabbit monoclonal [YE353] to PYK2
  • SpecificityThis antibody recognizes PYK2. It does not cross react with other FAK family members.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PYK2 aa 1-100 (N terminal).

  • Positive control
    • WB: Ramos, Jurkat and RAW264.7 cell lysates and mouse and rat brain tissue lysates. IHC-P: Human, mouse and rat cerebral cortex tissues. ICC/IF: HeLa and PC12 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab32571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).

For unpurified use at 1/1000 - 1/5000.

IHC-P 1/300.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

For unpurified use at 1/250 - 1/500.

ICC/IF 1/60.

For unpurified use at 1/100.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInvolved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
    • Tissue specificityMost abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
    • Cellular localizationCytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
    • Information by UniProt
    • Database links
    • Alternative names
      • CADTK antibody
      • CAK-beta antibody
      • CAKB antibody
      • CAKbeta antibody
      • Calcium regulated non receptor proline rich tyrosine kinase antibody
      • Calcium-dependent tyrosine kinase antibody
      • Cell adhesion kinase beta antibody
      • E430023O05Rik antibody
      • EC 2.7.10.2 antibody
      • FADK 2 antibody
      • FADK2 antibody
      • FAK2 antibody
      • FAK2_HUMAN antibody
      • Focal adhesion kinase 2 antibody
      • MGC124628 antibody
      • PKB antibody
      • Proline-rich tyrosine kinase 2 antibody
      • Protein kinase B antibody
      • Protein Tyrosine Kinase 2 Beta antibody
      • Protein-tyrosine kinase 2-beta antibody
      • PTK antibody
      • PTK2B antibody
      • PTK2B protein tyrosine kinase 2 beta antibody
      • PYK2 antibody
      • RAFTK antibody
      • RAFTK2 antibody
      • Related adhesion focal tyrosine kinase antibody
      see all

    Anti-PYK2 antibody [YE353] images

    • All lanes : Anti-PYK2 antibody [YE353] (ab32571) at 1/10000 dilution (purified)

      Lane 1 : Ramos cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 116 kDa
      Observed band size : 116 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-PYK2 antibody [YE353] (ab32571) at 10000 cells (purified) + RAW264.7 cell lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 116 kDa
      Observed band size : 116 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-PYK2 antibody [YE353] (ab32571) at 1/2000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 116 kDa
      Observed band size : 116 kDa

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-PYK2 antibody [YE353] (ab32571) at 1/5000 dilution (unpurified) + Jurkat cell lysate

      Predicted band size : 116 kDa
      Observed band size : 116 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling PYK2 with purified ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labelling PYK2 with purified ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebral cortex tissue labelling PYK2 with purified ab32571 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human brain tissue labelling PYK2 with unpurified ab32571 at a 1/250 dilution.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PYK2 with purified ab32571 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    • Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PYK2 with unpurified ab32571 at a 1/100 dilution.

    References for Anti-PYK2 antibody [YE353] (ab32571)

    This product has been referenced in:
    • Chu W  et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med 12:1741-1749 (2016). Read more (PubMed: 27602089) »
    • Gadepalli R  et al. Novel Role for p21-activated Kinase 2 in Thrombin-induced Monocyte Migration. J Biol Chem 288:30815-31 (2013). WB . Read more (PubMed: 24025335) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your kind reminder and your patience. I have been discussing this enquiry with some colleagues.
    From what we understand the customer is using this antibody (ab32571: Anti-PYK2 antibody) for cell staining, so I would recommend our ICC...

    Read More

    Thank you for your enquiry regarding ab32571 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.
    We have been selling this antibody without an...

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    Ja, Sie dürfen die Immunogen-Information gerne in Ihrer Publikation erwähnen.
    Ich würde Sie auch gerne bitten die volle Produktnummer (ab32571) anzugeben und uns vielleicht auch eine Kopie Ihrer Publikation zukommen zu lassen. Wir würden uns darüb...

    Read More

    Vielen Dank für Ihren Anruf.
    Es freut mich Ihnen nun die Region, in der das Immunogen liegt, genauer angeben zu können:
    Aminosäure 1 bis 30 von humanem PYK2.
    Ich kann Ihnen somit auch bestätigen, dass Aminosäure 405 nicht im Immunogen en...

    Read More

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