Anti-PYK2 (phospho Y579) antibody (ab193674)

Overview

  • Product name
    Anti-PYK2 (phospho Y579) antibody
    See all PYK2 primary antibodies
  • Description
    Rabbit polyclonal to PYK2 (phospho Y579)
  • Specificity
    ab193674 detects endogenous levels of PYK2 only when phosphorylated at tyrosine 579.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human PYK2 (phospho Y579) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence is around the phosphorylation site of tyrosine 579(E-D-Y(p)-Y-K).
    Database link: Q14289

  • Positive control
    • Human brain tissue; extracts from 3T3 and HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab193674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 119 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • Tissue specificity
    Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • Cellular localization
    Cytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADTK antibody
    • CAK-beta antibody
    • CAKB antibody
    • CAKbeta antibody
    • Calcium regulated non receptor proline rich tyrosine kinase antibody
    • Calcium-dependent tyrosine kinase antibody
    • Cell adhesion kinase beta antibody
    • E430023O05Rik antibody
    • EC 2.7.10.2 antibody
    • FADK 2 antibody
    • FADK2 antibody
    • FAK2 antibody
    • FAK2_HUMAN antibody
    • Focal adhesion kinase 2 antibody
    • MGC124628 antibody
    • PKB antibody
    • Proline-rich tyrosine kinase 2 antibody
    • Protein kinase B antibody
    • Protein Tyrosine Kinase 2 Beta antibody
    • Protein-tyrosine kinase 2-beta antibody
    • PTK antibody
    • PTK2B antibody
    • PTK2B protein tyrosine kinase 2 beta antibody
    • PYK2 antibody
    • RAFTK antibody
    • RAFTK2 antibody
    • Related adhesion focal tyrosine kinase antibody
    see all

Images

  • All lanes : Anti-PYK2 (phospho Y579) antibody (ab193674) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells treated with antigen-specific peptide
    Lane 2 : Extracts from 3T3 cells
    Lane 3 : Extracts from HepG2 cells


    Predicted band size : 119 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue sections labeling PYK2 (phospho Y579) with ab193674 at a 1/50 dilution. Right image was preincubated with blocking peptide.

References

ab193674 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193674.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up