Anti-PYK2 (phospho Y580) antibody (ab52209)


  • Product nameAnti-PYK2 (phospho Y580) antibody
    See all PYK2 primary antibodies
  • Description
    Rabbit polyclonal to PYK2 (phospho Y580)
  • Specificityab52209 detects endogenous levels of PYK2 only when phosphorylated at tyrosine 580.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human PYK2 around the phosphorylation site of tyrosine 580 (D-Y-YP-K-A).

  • Positive control
    • Human brain tissue, extracts from K562 cells.



Our Abpromise guarantee covers the use of ab52209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.


  • FunctionInvolved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. In concert with SRC, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. The Tyr-402 phosphorylated form serves as a docking site for SRC and is important for the organization of the osteoclast actin cytoskeleton and attachment sites and for bone resorption.
  • Tissue specificityMost abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha-lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity.
  • Cellular localizationCytoplasm. Cell membrane. Interaction with nephrocystin induces the membrane-association of the kinase.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADTK antibody
    • CAK-beta antibody
    • CAKB antibody
    • CAKbeta antibody
    • Calcium regulated non receptor proline rich tyrosine kinase antibody
    • Calcium-dependent tyrosine kinase antibody
    • Cell adhesion kinase beta antibody
    • E430023O05Rik antibody
    • EC antibody
    • FADK 2 antibody
    • FADK2 antibody
    • FAK2 antibody
    • FAK2_HUMAN antibody
    • Focal adhesion kinase 2 antibody
    • MGC124628 antibody
    • PKB antibody
    • Proline-rich tyrosine kinase 2 antibody
    • Protein kinase B antibody
    • Protein Tyrosine Kinase 2 Beta antibody
    • Protein-tyrosine kinase 2-beta antibody
    • PTK antibody
    • PTK2B antibody
    • PTK2B protein tyrosine kinase 2 beta antibody
    • PYK2 antibody
    • RAFTK antibody
    • RAFTK2 antibody
    • Related adhesion focal tyrosine kinase antibody
    see all

Anti-PYK2 (phospho Y580) antibody images

  • This image shows human brain tissue stained with ab52209 at a dilution of 1/50 - 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated.
  • All lanes : Anti-PYK2 (phospho Y580) antibody (ab52209) at 1/500 dilution

    Lane 1 : Extracts from K562 cells with no immunising peptide
    Lane 2 : Extracts from K562 cells with immunising peptide

    Predicted band size : 116 kDa

References for Anti-PYK2 (phospho Y580) antibody (ab52209)

ab52209 has not yet been referenced specifically in any publications.

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Ich habe die Testdatendes Antikörpers in ELISA mit dem Phospho-Peptid und dem un-phosphorylierten Peptid als Anhang zugefügt. ab52209 zeigt eine deutl...

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