Overview

  • Product name
  • Description
    Rabbit polyclonal to Pyrin
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Pyrin aa 268-284 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    AANLDSATEPRARPTPD

  • Positive control
    • Human kidney tissue; Human spleen tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.

Target

  • Function
    Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization.
  • Tissue specificity
    Expressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines.
  • Involvement in disease
    Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence.
    Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness.
  • Sequence similarities
    Contains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 DAPIN domain.
  • Developmental stage
    First detected in bone marrow promyelocytes. Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells.
  • Cellular localization
    Nucleus and Cytoplasm > cytoskeleton. Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FMF antibody
    • Marenostrin antibody
    • Mediterranean fever antibody
    • Mediterranean fever protein antibody
    • MEF antibody
    • Mefv antibody
    • MEFV_HUMAN antibody
    • Pyrin antibody
    • TRIM20 antibody
    see all

Images

  • ab115331 at 10ug/ml staining MEFV in Human kidney tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115331 at 10ug/ml staining MEFV in Human spleen tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115331 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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