• Product nameAnti-Pyrin antibody
    See all Pyrin primary antibodies
  • Description
    Rabbit polyclonal to Pyrin
  • SpecificityThis antibody recognizes MEFV
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Pyrin aa 1-50 (N terminal).


  • Positive control
    • HepG2 cell lysate



Our Abpromise guarantee covers the use of ab49121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.625 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:62500.


  • FunctionProbably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization.
  • Tissue specificityExpressed in peripheral blood leukocytes, particularly in mature granulocytes and to a lesser extent in monocytes but not in lymphocytes. Detected in spleen, lung and muscle, probably as a result of leukocyte infiltration in these tissues. Not expressed in thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, liver, kidney, pancreas. Expression detected in several myeloid leukemic, colon cancer, and prostate cancer cell lines.
  • Involvement in diseaseDefects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence.
    Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness.
  • Sequence similaritiesContains 1 B box-type zinc finger.
    Contains 1 B30.2/SPRY domain.
    Contains 1 DAPIN domain.
  • Developmental stageFirst detected in bone marrow promyelocytes. Expression increases throughout myelocyte differentiation and peaks in the mature myelomonocytic cells.
  • Cellular localizationNucleus and Cytoplasm > cytoskeleton. Associated with microtubules and with the filamentous actin of perinuclear filaments and peripheral lamellar ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • FMF antibody
    • Marenostrin antibody
    • Mediterranean fever antibody
    • Mediterranean fever protein antibody
    • MEF antibody
    • Mefv antibody
    • MEFV_HUMAN antibody
    • Pyrin antibody
    • TRIM20 antibody
    see all

Anti-Pyrin antibody images

  • Lane 1 : Marker
    Lane 2 : Anti-Pyrin antibody (ab49121) at 0.625 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg with 5% skimmed milk in PBS.

    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Gel concentration 12%

References for Anti-Pyrin antibody (ab49121)

ab49121 has not yet been referenced specifically in any publications.

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Thank you for for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that your customer...

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Thank you fro clarifying that information.

Having reviewed the protocol provided by your customer there are a few suggestions I can make in order to improve the performance of anti-MEFV antibody (ab49121).

1. Typically 1...

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Thank you fro contacting us and reporting the problems your customer has been experiencing with Anti-MEFV antibody (ab49121).

In order to understand this case a little further, could you please clarify what the 1% TBSTsolution is made u...

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Thank you for contacting us. We have no FACS data for detection of MEFV, or data from other applications that detect MEFV in its native conformation, only western blot detection of denatured samples and ELISA against the immunogen peptide. Thes...

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