Overview

  • Product name
    Pyronin Y (Pyronin G)
  • Description
    Intercalating cationic dye with specificity for RNA.
  • Alternative names
    • Pyronin G
    • Pyronin J
    • Pyronin Y
  • Biological description

    Cationic dye that intercalates RNA and is visible as a red band during electrophoresis. Shown to accumulate in the mitochondria of viable cells. At low concentrations the cytostatic effect is cell arrest in the G1 phase, whereas at high concentrations the cytotoxic effect is cell arrest in the G2 and S phase. Used in combination with Alcian Blue to assess and study pulmonary diseases, and in combination with Methyl Green to stain nucleic acids in paraffin sections. Additionally, has been used to measure RNA in CHO cells by flow cytometry.

Properties

  • Chemical name
    N-[6-(Dimethylamino)-3H-xanthen-3-ylidene]-N-methylmethanaminium chloride
  • Molecular weight
    302.80
  • Chemical structure
    Chemical Structure
  • Molecular formula
    C17H19ClN2O
  • CAS Number
    92-32-0
  • Storage instructions
    Store at Room Temperature. The product can be stored for up to 12 months.
  • Solubility overview
    Soluble in water to 75 mM and in ethanol to 10 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:
  • Chusri S  et al. Enhancing antibiotic activity: a strategy to control Acinetobacter infections. J Antimicrob Chemother 64:1203-11 (2009). Read more (PubMed: 19861335) »
  • Karpinska-Kaczmarczyk K  et al. Prognostic significance of morphometric parameters of nucleoli and nuclei of invasive ductal breast carcinomas. Pol J Pathol 60:124-9 (2009). Read more (PubMed: 20069505) »
  • Meyerholz DK  et al. Alcian Blue and Pyronine Y histochemical stains permit assessment of multiple parameters in pulmonary disease models. Vet Pathol 46:325-8 (2009). Read more (PubMed: 19261646) »
  • Prentø P & Lyon HO Methyl green-pyronin Y staining of nucleic acids: studies on the effects of staining time, dye composition and diffusion rates. Biotech Histochem 78:27-33 (2003). Read more (PubMed: 12713139) »
  • Darzynkiewicz Z  et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res 46:5760-6 (1986). Read more (PubMed: 2428484) »
  • Crissman HA  et al. Normal and perturbed Chinese hamster ovary cells: correlation of DNA, RNA, and protein content by flow cytometry. J Cell Biol 101:141-7 (1985). Read more (PubMed: 2409095) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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