Overview

  • Product nameAnti-Pyrophosphatase 1 antibody
    See all Pyrophosphatase 1 primary antibodies
  • Description
    Rabbit polyclonal to Pyrophosphatase 1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Cow, Monkey
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 259 of Human Pyrophosphatase 1 (UniProt ID: Q15181).

  • Positive control
    • 293T, Raji. A431, H1299, HeLa, HepG2, MOLT4 whole cell lysates for WB. HeLa cells for IF. OVCAR3 xenograft for IHC.

Properties

Applications

Our Abpromise guarantee covers the use of ab96099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

Anti-Pyrophosphatase 1 antibody images

  • All lanes : Anti-Pyrophosphatase 1 antibody (ab96099) at 1/10000 dilution

    Lane 1 : H1299 Whole cell lysate
    Lane 2 : HeLa Whole cell lysate
    Lane 3 : HepG2 Whole cell lysate
    Lane 4 : MOLT4 Whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 32 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96099 at 1/200 dilution. Lower image merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ab96099 at 1/500 dilution.

References for Anti-Pyrophosphatase 1 antibody (ab96099)

This product has been referenced in:
  • Megger DA  et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). Read more (PubMed: 23462207) »
  • Yi YJ  et al. Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa. PLoS One 7:e34524 (2012). WB, ICC/IF ; Pig . Read more (PubMed: 22485177) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"