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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
1/100 - 1/500.
1/100 - 1/200.
Tissue specificity Expressed ubiquitously.
Sequence similarities Belongs to the PPase family.
Cellular localization Cytoplasm.
Information by UniProt
cytosolic inorganic pyrophosphatase antibody
diphosphate phosphohydrolase antibody
inorganic diphosphatase antibody
Anti-Pyrophosphatase 1 antibody images
Western blot - Pyrophosphatase 1 antibody (ab96099)
All lanes : Anti-Pyrophosphatase 1 antibody (ab96099) at 1/10000 dilution Lane 1 : H1299 Whole cell lysate Lane 2 : HeLa Whole cell lysate Lane 3 : HepG2 Whole cell lysate Lane 4 : MOLT4 Whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size : 32 kDa
Immunocytochemistry/ Immunofluorescence - Pyrophosphatase 1 antibody (ab96099)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96099 at 1/200 dilution. Lower image merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pyrophosphatase 1 antibody (ab96099)
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ab96099 at 1/500 dilution.
References for Anti-Pyrophosphatase 1 antibody (ab96099)
This product has been referenced in:
et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013).
Read more (PubMed: 23462207) »
et al. Identification of the inorganic pyrophosphate metabolizing, ATP substituting pathway in mammalian spermatozoa. PLoS One 7:e34524 (2012).
Read more (PubMed: 22485177) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"