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Pyrophosphates (PPi) are produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.
Pyrophosphate Assay Kit (Fluorometric) provides the most robust spectrophotometric method for the measurement of pyrophosphate. It uses a proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Abcam's assay is much easier and more robust than enzyme-coupling pyrophosphate methods, which require at least two enzymes for their pyrophosphate detections. Due to its direct measurement of pyrophosphate, this kit is ideal for screening inhibition or activities of enzymes that consume or generate pyrophosphate. The assay is an optimized mix-and-read assay and can be performed in a convenient 96-well or 384-well microtiter-plate format. The kit provides all the essential components for assaying pyrophosphate.
Visit our FAQs page for tips and troubleshooting.
This product measures PPi formation at Ex/Em = 316/456 nm.
If your instrument has filters for Ex/Em = 370/470 nm, we recommend using Pyrophosphate Assay Kit II (Fluorometric) (ab179836).
|Components||2 x 96 tests|
|Assay Buffer||1 x 25ml|
|DMSO||1 x 200µl|
|PPi Sensor||1 vial|
|Pyrophosphate Standard (50mM)||1 x 1ml|
Our Abpromise guarantee covers the use of ab112155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Urine PPi was corrected for urine creatinine, which was measured by LC-MS/MS or by ELISA using appropriate controls to adjust for inter-assay variability.
Pyrophospfate measured in cell lysates showing quantity (nmol) per 106cells
Pyrophosphate measured in tissue lysates showing quantity (nmol) per microgram protein
Pyrophosphate measured in biofluids showing concentration (micromolar). Obtained values were recalculated from 1:1000 sample dilutions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"