Full length native protein (purified) corresponding to Human Pyruvate Dehydrogenase E1-alpha subunit.
Database link: P08559
Product was previously marketed under the MitoSciences sub-brand.
Our Abpromise guarantee covers the use of ab110334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 25223649|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 43 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml. (heat-induced antigen-retrieval improves signal)|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunocytochemistry analysis using ab110334 at 1 µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in HeLa (human epithelial cell line from cervix adenocarcinoma) cells (4% paraformaldehyde fixed and Triton X-100 permeabilized).
The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis using ab110334 at 1 µg/ml staining Pyruvate Dehydrogenase E1-alpha subunit in HL-60 (human promyelocytic leukemia cell line) cells (blue). Isotype control antibody (red).