Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Pyruvate Dehydrogenase E1-alpha subunit antibody
See all Pyruvate Dehydrogenase E1-alpha subunit primary antibodies -
DescriptionMouse polyclonal to Pyruvate Dehydrogenase E1-alpha subunit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Full length PDHA1 protein (Human)
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Positive control
- Human liver tissue lysate, PDHA1 transfected lysate and Human stomach tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab67592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). | |
| IHC-P | Use a concentration of 1.5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
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Tissue specificityUbiquitous.
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Involvement in diseaseDefects in PDHA1 are a cause of pyruvate decarboxylase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (X-LS).
Defects in PDHA1 are the cause of X-linked Leigh syndrome (X-LS) [MIM:308930]. X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord. The lesions are areas of demyelination, gliosis, necrosis, spongiosis, or capillary proliferation. Clinical symptoms depend on which areas of the central nervous system are involved. The most common underlying cause is a defect in oxidative phosphorylation. LS may be a feature of a deficiency of any of the mitochondrial respiratory chain complexes. -
Cellular localizationMitochondrion matrix.
- Information by UniProt
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Database links
- Entrez Gene: 5160 Human
- Entrez Gene: 18597 Mouse
- Omim: 300502 Human
- SwissProt: P08559 Human
- SwissProt: P35486 Mouse
- Unigene: 530331 Human
- Unigene: 34775 Mouse
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Alternative names
- ODPA_HUMAN antibody
- PDH antibody
- PDHA antibody
see all
Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody images
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Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution + Human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 43 kDa -
Western blot - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)All lanes : Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592) at 1/500 dilution
Lane 1 : Pyruvate Dehydrogenase E1-alpha subunit transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)Immunohistochemical analysis of formalin fixed and paraffin embedded human stomach tissue labelled with ab67592 at 1.5µg/ml. -
Immunocytochemistry/ Immunofluorescence-Pyruvate Dehydrogenase E1-alpha subunit antibody(ab67592)ICC/IF image of ab67592 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67592, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References for Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (ab67592)
This product has been referenced in:
- Chung J et al. Erythropoietin signaling regulates heme biosynthesis. Elife 6:N/A (2017). Read more (PubMed: 28553927) »
- Chung J et al. The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability. Sci Signal 8:ra34 (2015). WB . Read more (PubMed: 25872869) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"