Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab126203)

Overview

  • Product name
    Anti-Pyruvate Dehydrogenase E1 beta subunit antibody
    See all Pyruvate Dehydrogenase E1 beta subunit primary antibodies
  • Description
    Rabbit polyclonal to Pyruvate Dehydrogenase E1 beta subunit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 78-335 of Human Pyruvate Dehydrogenase E1 beta subunit (P11177).

  • Positive control
    • A549, H1299, HCT116 and Mouse heart whole cell lysates; U87 xenograft tissue. IF/ICC: HepG2 cell line.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 1 µg/ml.

GR88682-3

Target

  • Function
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in disease
    Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Images

  • ICC/IF image of ab126203 stained HepG2 cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126203, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab126203) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
    10% SDS PAGE
  • Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab126203) at 1/10000 dilution + Mouse heart whole cell lysate at 50 µg

    Predicted band size : 39 kDa
    10% SDS PAGE
  • ab126203, at 1/500 dilution, staining Pyruvate Dehydrogenase E1 beta subunit in paraffin-embedded U87 xenograft tissue by Immunohistochemistry.

References

ab126203 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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