Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab84588)

Overview

  • Product nameAnti-Pyruvate Dehydrogenase E1 beta subunit antibody
    See all Pyruvate Dehydrogenase E1 beta subunit primary antibodies
  • Description
    Rabbit polyclonal to Pyruvate Dehydrogenase E1 beta subunit
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (GLWKKYGDKR IIDTPISEMG FAGIAVGAAM AGLRPICEFM TFNFSMQAID) of Human Pyruvate Dehydrogenase E1 beta subunit (NP_000916).

  • Positive control
    • Human fetal heart lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in diseaseDefects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp564K0164 antibody
    • mitochondrial antibody
    • ODPB_HUMAN antibody
    • pdhB antibody
    • PDHBD antibody
    • PDHE1 B antibody
    • PDHE1-B antibody
    • PHE1B antibody
    • Pyruvate dehydrogenase (lipoamide) beta antibody
    • Pyruvate dehydrogenase E1 beta polypeptide antibody
    • Pyruvate dehydrogenase E1 component subunit beta antibody
    • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
    see all

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody images

  • Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab84588) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References for Anti-Pyruvate Dehydrogenase E1 beta subunit antibody (ab84588)

ab84588 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
As long as your mutant construct contains the either amino acid regions 72-121 or 250-360, then you should be able to either ab84588 orab55574, as these antibodies will bind within these regions respectively.
Whether...

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Thank you for contacting Abcam.
Depending on the exact amino acids that are missing from your mutant construct, one of the Pyruvate Dehydrogenase E1 beta subunit antibodies we have should work for you. Below is the immunogen information for each of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"