Recombinant
RabMAb

Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11096(B)] (ab155953)

Overview

  • Product name
    Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11096(B)]
    See all Pyruvate Dehydrogenase E1 beta subunit primary antibodies
  • Description
    Rabbit monoclonal [EPR11096(B)] to Pyruvate Dehydrogenase E1 beta subunit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues within Human Pyruvate Dehydrogenase E1 beta subunit (UniProt: P11177).

  • Positive control
    • HepG2, HeLa, A375 and Human fetal heart lysates; Human colon and pancreas tissues; HepG2 and A375 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab155953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
    • Involvement in disease
      Defects in PDHB are a cause of pyruvate dehydrogenase E1 component deficiency (PDHE1 deficiency) [MIM:312170]. PDHE1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. It is associated with variable clinical phenotypes ranging from neonatal death to prolonged survival complicated by developmental delay, seizures, ataxia, apnea, and in some cases to an X-linked form of Leigh syndrome (LS) (Leigh encephalomyelopathy).
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp564K0164 antibody
      • mitochondrial antibody
      • ODPB_HUMAN antibody
      • pdhB antibody
      • PDHBD antibody
      • PDHE1 B antibody
      • PDHE1-B antibody
      • PHE1B antibody
      • Pyruvate dehydrogenase (lipoamide) beta antibody
      • Pyruvate dehydrogenase E1 beta polypeptide antibody
      • Pyruvate dehydrogenase E1 component subunit beta antibody
      • Pyruvate dehydrogenase E1 component subunit beta mitochondrial antibody
      see all

    Images

    • ab155953 showing +ve staining in Human thyroid gland carcinoma.
    • ab155953 showing +ve staining in Human ovarian carcinoma.
    • ab155953 showing +ve staining in Human heart.
    • ab155953 showing +ve staining in Human gastric adenocarcinoma.
    • All lanes : Anti-Pyruvate Dehydrogenase E1 beta subunit antibody [EPR11096(B)] (ab155953) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal heart tissue lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 39 kDa
    • Immunofluorescent analysis of HepG2 cells, labeling Pyruvate Dehydrogenase E1 beta subunit with ab155953 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling Pyruvate Dehydrogenase E1 beta subunit with ab155953 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue, labeling Pyruvate Dehydrogenase E1 beta subunit with ab155953 at 1/50 dilution.
    • Flow cytometric analysis of permeabilized A375 cells labeling Pyruvate Dehydrogenase E1 beta subunit with ab155953 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

    References

    ab155953 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab155953.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up