Anti-Pyruvate Dehydrogenase E2 antibody (ab126224)
Key features and details
- Rabbit polyclonal to Pyruvate Dehydrogenase E2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-Pyruvate Dehydrogenase E2 antibody
See all Pyruvate Dehydrogenase E2 primary antibodies -
Description
Rabbit polyclonal to Pyruvate Dehydrogenase E2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 310-548 of Human Pyruvate Dehydrogenase E2 (UniProt ID: P10515).
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Positive control
- 293T, A431, HeLa and HepG2 whole cell lysate (ab7900)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/5000 - 1/20000. Predicted molecular weight: 69 kDa.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 69 kDa. |
Target
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Function
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). -
Involvement in disease
Note=Primary biliary cirrhosis is a chronic, progressive cholestatic liver disease characterized by the presence of antimitochondrial autoantibodies in patients' serum. It manifests with inflammatory obliteration of intra-hepatic bile duct, leading to liver cell damage and cirrhosis. Patients with primary biliary cirrhosis show autoantibodies against the E2 component of pyruvate dehydrogenase complex.
Defects in DLAT are the cause of pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency) [MIM:245348]; also known as lactic acidemia due to defect of E2 lipoyl transacetylase of the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (PDH) deficiency is a major cause of primary lactic acidosis and neurological dysfunction in infancy and early childhood. In this form of PDH deficiency episodic dystonia is the major neurological manifestation, with other more common features of pyruvate dehydrogenase deficiency, such as hypotonia and ataxia, being less prominent. -
Sequence similarities
Belongs to the 2-oxoacid dehydrogenase family.
Contains 2 lipoyl-binding domains. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 1737 Human
- Omim: 608770 Human
- SwissProt: P10515 Human
- Unigene: 335551 Human
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Alternative names
- 70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody
- Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody
- Dihydrolipoamide S Acetyltransferase antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab126224 has been referenced in 3 publications.
- Hossain AJ et al. Pyruvate Dehydrogenase A1 Phosphorylated by Insulin Associates with Pyruvate Kinase M2 and Induces LINC00273 through Histone Acetylation. Biomedicines 10:N/A (2022). PubMed: 35740278
- Ward NP et al. Nicotinamide nucleotide transhydrogenase regulates mitochondrial metabolism in NSCLC through maintenance of Fe-S protein function. J Exp Med 217:N/A (2020). PubMed: 32196080
- Osinalde N et al. Changes in Gab2 phosphorylation and interaction partners in response to interleukin (IL)-2 stimulation in T-lymphocytes. Sci Rep 6:23530 (2016). WB . PubMed: 27025927