Anti-Pyruvate Dehydrogenase E2 antibody (ab66511)

Overview

  • Product nameAnti-Pyruvate Dehydrogenase E2 antibody
    See all Pyruvate Dehydrogenase E2 primary antibodies
  • Description
    Rabbit polyclonal to Pyruvate Dehydrogenase E2
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Cat, Pig, Zebrafish
  • Immunogen

    Synthetic peptide: LLQLLGSPGR RYYSLPPHQK VPLPSLSPTM QAGTIARWEK KEGDKINEGD, corresponding to a region within N terminal amino acids 74-123 of Human Pyruvate Dehydrogenase E2

  • Positive control
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab66511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml.
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in diseaseNote=Primary biliary cirrhosis is a chronic, progressive cholestatic liver disease characterized by the presence of antimitochondrial autoantibodies in patients' serum. It manifests with inflammatory obliteration of intra-hepatic bile duct, leading to liver cell damage and cirrhosis. Patients with primary biliary cirrhosis show autoantibodies against the E2 component of pyruvate dehydrogenase complex.
    Defects in DLAT are the cause of pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency) [MIM:245348]; also known as lactic acidemia due to defect of E2 lipoyl transacetylase of the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (PDH) deficiency is a major cause of primary lactic acidosis and neurological dysfunction in infancy and early childhood. In this form of PDH deficiency episodic dystonia is the major neurological manifestation, with other more common features of pyruvate dehydrogenase deficiency, such as hypotonia and ataxia, being less prominent.
  • Sequence similaritiesBelongs to the 2-oxoacid dehydrogenase family.
    Contains 2 lipoyl-binding domains.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody
    • Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody
    • Dihydrolipoamide S Acetyltransferase antibody
    • Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody
    • dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial antibody
    • DLAT antibody
    • DLTA antibody
    • E2 antibody
    • E2 component of pyruvate dehydrogenase complex antibody
    • M2 antigen complex 70 kDa subunit antibody
    • mitochondrial antibody
    • ODP2_HUMAN antibody
    • PBC antibody
    • PDC E2 antibody
    • PDC-E2 antibody
    • PDCE2 antibody
    • Pyruvate dehydrogenase complex component E2 antibody
    • Pyruvate dehydrogenase complex E2 subunit antibody
    see all

Anti-Pyruvate Dehydrogenase E2 antibody images

  • Immunochemistry/Immunofluorescence analysis of Hek293 cell labelling Pyruvate Dehydrogenase E2 with ab66511 at 1/100 dilution. An anti-rabbit Alexa Fluor 488 was used as the secondary antibody at 1/400 dilution. Mitotraker-Red was used to stain mitochondria and DAPI was used to stain nuclei.

    See Abreview

  • Anti-Pyruvate Dehydrogenase E2 antibody (ab66511) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Pyruvate Dehydrogenase E2 antibody (ab66511) at 1/2500 dilution (For 16 hours in blocking solution.)

    Lane 1 : Mouse adipocyte whole cell lysate
    Lane 2 : Mouse adipocyte whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 66 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at 25°C.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pyruvate Dehydrogenase E2 with ab66511 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

  • Anti-Pyruvate Dehydrogenase E2 antibody (ab66511) at 1/200 dilution + Huh7 cell lysate at 50 µg

    Predicted band size : 66 kDa
    WB Suggested Anti-TSPAN3 antibody (ab81018) Titration: 1 µg/ml Positive Control: sea urchin embryo

References for Anti-Pyruvate Dehydrogenase E2 antibody (ab66511)

This product has been referenced in:
  • Miller JA  et al. 1,3-Dinitrobenzene-induced metabolic impairment through selective inactivation of the pyruvate dehydrogenase complex. Toxicol Sci 122:502-11 (2011). WB ; Rat . Read more (PubMed: 21551353) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Antigen retrieval step None
Sample Mouse Tissue sections (mouse heart)
Specification mouse heart
Permeabilization Yes - 1%triton x-100
Fixative Acetone
Username

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Verified customer

Submitted Jun 11 2013

Merci pour votre requête.

Nous n'avonsmalheureusementpas d'échantillons ou de petit volume de nos produits. Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique, nous offrons...

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Thank you for your inquiry. The antibodies (ab6078, ab8609, ab20034, ab22683, ab32575, ab66511, ab74715) need an MSDS only for the Sodium Azide in the storage buffer. For ab66511 there is no MSDS neccessary at all. The MSDS can be found on the da...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Adipocyte)
Loading amount 10 µg
Specification Adipocyte
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"