Anti-Pyruvate Dehydrogenase E2 antibody (ab88884)

Overview

  • Product nameAnti-Pyruvate Dehydrogenase E2 antibody
    See all Pyruvate Dehydrogenase E2 primary antibodies
  • Description
    Mouse polyclonal to Pyruvate Dehydrogenase E2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human Pyruvate Dehydrogenase E2 (NP_001922.2)

  • Positive control
    • Rat brain tissue lysate, HepG2 cell lysate, Pyruvate Dehydrogenase E2 transfected 293T cell lysate and HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Involvement in diseaseNote=Primary biliary cirrhosis is a chronic, progressive cholestatic liver disease characterized by the presence of antimitochondrial autoantibodies in patients' serum. It manifests with inflammatory obliteration of intra-hepatic bile duct, leading to liver cell damage and cirrhosis. Patients with primary biliary cirrhosis show autoantibodies against the E2 component of pyruvate dehydrogenase complex.
    Defects in DLAT are the cause of pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency) [MIM:245348]; also known as lactic acidemia due to defect of E2 lipoyl transacetylase of the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (PDH) deficiency is a major cause of primary lactic acidosis and neurological dysfunction in infancy and early childhood. In this form of PDH deficiency episodic dystonia is the major neurological manifestation, with other more common features of pyruvate dehydrogenase deficiency, such as hypotonia and ataxia, being less prominent.
  • Sequence similaritiesBelongs to the 2-oxoacid dehydrogenase family.
    Contains 2 lipoyl-binding domains.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody
    • Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody
    • Dihydrolipoamide S Acetyltransferase antibody
    • Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody
    • dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial antibody
    • DLAT antibody
    • DLTA antibody
    • E2 antibody
    • E2 component of pyruvate dehydrogenase complex antibody
    • M2 antigen complex 70 kDa subunit antibody
    • mitochondrial antibody
    • ODP2_HUMAN antibody
    • PBC antibody
    • PDC E2 antibody
    • PDC-E2 antibody
    • PDCE2 antibody
    • Pyruvate dehydrogenase complex component E2 antibody
    • Pyruvate dehydrogenase complex E2 subunit antibody
    see all

Anti-Pyruvate Dehydrogenase E2 antibody images

  • Anti-Pyruvate Dehydrogenase E2 antibody (ab88884) at 1 µg/ml + Rat brain tissue lysate at 50 µg

    Predicted band size : 66 kDa
    Observed band size : 66 kDa
  • Anti-Pyruvate Dehydrogenase E2 antibody (ab88884) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size : 66 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Pyruvate Dehydrogenase E2 antibody (ab88884) at 1 µg/ml

    Lane 1 : Pyruvate Dehydrogenase E2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ab88884 at 10 ug/ml staining Pyruvate Dehydrogenase E2 in HeLa cells by Immunofluoresence.

References for Anti-Pyruvate Dehydrogenase E2 antibody (ab88884)

ab88884 has not yet been referenced specifically in any publications.

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