Recombinant
RabMAb

Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617)

Overview

  • Product name
    Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)]
    See all Pyruvate Dehydrogenase E2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12455(B)] to Pyruvate Dehydrogenase E2
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pyruvate Dehydrogenase E2 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P10515

  • Positive control
    • HeLa, HepG2, Jurkat and 293T cell lysates; HepG2 and Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab172617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
    • Involvement in disease
      Note=Primary biliary cirrhosis is a chronic, progressive cholestatic liver disease characterized by the presence of antimitochondrial autoantibodies in patients' serum. It manifests with inflammatory obliteration of intra-hepatic bile duct, leading to liver cell damage and cirrhosis. Patients with primary biliary cirrhosis show autoantibodies against the E2 component of pyruvate dehydrogenase complex.
      Defects in DLAT are the cause of pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency) [MIM:245348]; also known as lactic acidemia due to defect of E2 lipoyl transacetylase of the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (PDH) deficiency is a major cause of primary lactic acidosis and neurological dysfunction in infancy and early childhood. In this form of PDH deficiency episodic dystonia is the major neurological manifestation, with other more common features of pyruvate dehydrogenase deficiency, such as hypotonia and ataxia, being less prominent.
    • Sequence similarities
      Belongs to the 2-oxoacid dehydrogenase family.
      Contains 2 lipoyl-binding domains.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • 70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody
      • Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody
      • Dihydrolipoamide S Acetyltransferase antibody
      • Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody
      • dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial antibody
      • DLAT antibody
      • DLTA antibody
      • E2 antibody
      • E2 component of pyruvate dehydrogenase complex antibody
      • M2 antigen complex 70 kDa subunit antibody
      • mitochondrial antibody
      • ODP2_HUMAN antibody
      • PBC antibody
      • PDC E2 antibody
      • PDC-E2 antibody
      • PDCE2 antibody
      • Pyruvate dehydrogenase complex component E2 antibody
      • Pyruvate dehydrogenase complex E2 subunit antibody
      see all

    Images

    • All lanes : Anti-Pyruvate Dehydrogenase E2 antibody [EPR12455(B)] (ab172617) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 69 kDa
    • Immunofluorescent analysis of HepG2 cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/50 dilution.

    • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab172617 at 1/10 dilution.

    • Flow cytometric analysis of permeabilized Jurkat cells labeling Pyruvate Dehydrogenase E2 with ab172617 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

    References

    ab172617 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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