Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882)

  Functional Studies - Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882)

Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

  - Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882)

Figure 1. An example using the ab109882 to measure PDH activity in human fibroblast sample. Developed dipsticks from a 1:2 dilution series using a positive control sample and the associated standard curve. Starting material was 160 µg of fibroblast protein extract in a 100 µL reaction volume (50 µL sample in Sample buffer plus 50 µL of Blocking solution). The dipsticks were developed for 1 hour in Activity buffer.

  - Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882)

Figure 2. An example using the ab109882 to measure PDH activity in human fibroblast sample. Based on the standard curve, 160 µg of protein extract were loaded onto a dipstick for each sample. The figure shows two developed dipsticks, a control sample and a sample from a patient with a mutation in the E1-alpha subunit of PDH. The analysis of the signal intensity and interpolation from the standard curve showed that the patient sample had 8% the PDH activity of the control.