ab109883 (MSP31) is used to quantify the activity of the PDH enzyme complex (pyruvate dehydrogenase) from human, bovine, mouse, and rat samples.
PDH is present in all tissues; it plays a central role in metabolism as it is a key regulatory enzyme that functions at the junction between glycolysis and the tricarboxylic acid cycle. The PDH complex is composed of multiple copies of three catalytic component enzymes; pyruvate dehydrogenase or E1 (EC 220.127.116.11), dihydrolipoamide transacetylase or E2 (EC 18.104.22.168) and dihydrolipoamide dehydrogenase or E3 (EC 22.214.171.124). Inherent to its regulatory function, a number of other proteins regulate PDH activity. One of these, dihydrolipoamide dehydrogenase-binding protein (E3Bp) is necessary for the interaction of the E2 and E3 components.
This dipstick assay kit utilizes two monoclonal antibodies. One recognizes the E2 enzyme (monoclonal antibody bound to the dipstick) and the other antibody (gold-conjugated) recognizes both the E2 and E3Bp components of the PDH complex. The signal intensity is measured by a dipstick reader or analyzed by other imaging systems such as a flatbed scanner. The kit is compatible with a variety of sample types (tissue or cell culture) from a number of different species (human, bovine, mouse, and rat).
All components are stable in their provided containers at room temperature out of direct sunlight.
After diluting the 10X Blocking Buffer to 2X, store at 4°C.
For long-term storage, all buffers can be stored at 4°C.
|Components||90 tests||30 tests|
|Buffer B (10X Blocking solution)||3 x 0.4ml||1 x 0.4ml|
|Dipsticks||1 x 90 units||1 x 30 units|
|Extraction Buffer||3 x 15ml||1 x 15ml|
|Gold-conjugated antibody (dried in microplate wells)||1 x 90 tests||1 x 30 tests|
|Wash buffer||3 x 1ml||1 x 1ml|
Our Abpromise guarantee covers the use of ab109883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab109883 has not yet been referenced specifically in any publications.