Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)

Overview

  • Product name
    Pyruvate dehydrogenase (PDH) WB Antibody Cocktail
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.

    The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.

    Cocktail Antibodies:

    Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
    Amount: 12.5 µg
    Working concentration: 0.5 µg/ml

    Mouse anti PDH-E1-alpha [9H9AF5] monoclonal:
    Amount: 25 µg
    Working concentration: 1 µg/ml

    Mouse anti PDH-E1-beta [17A5E2H8] monoclonal:
    Amount: 62.5 µg
    Working concentration: 2.5 µg/ml

    Mouse anti PDH-E2 [15D3G9C11] monoclonal:
    Amount: 25 µg
    Working concentration: 1 µg/ml

    Mouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
    Amount: 25 µg
    Working concentration: 1 µg/ml

  • Tested applications
    Suitable for: WBmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab110416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 6 µg/ml. Suggested concentration.

Images

  • Blocked for overnight with 5% Milk/PBS at 4ºC (or 3 hrs at room temperature).

    Primary antibody incubatedat 6 µg/mL in 1% milk/PBS for 2hrs at room temperature.

    Secondary: HRP-conjugated anti-mouse used according to manufacturers instructions.

    Detection method: ECL+

     

    All wash steps carried out in PBS + 0.05% Tween-20.

References

This product has been referenced in:
  • Naseri NN  et al. Novel Metabolic Abnormalities in the Tricarboxylic Acid Cycle in Peripheral Cells From Huntington's Disease Patients. PLoS One 11:e0160384 (2016). WB . Read more (PubMed: 27611087) »
  • Ferdinandusse S  et al. Clinical and biochemical characterization of four patients with mutations in ECHS1. Orphanet J Rare Dis 10:79 (2015). WB ; Human . Read more (PubMed: 26081110) »

See all 9 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Please find the WB protocol used to validate our MitoSciences range of antibodies attached, as well as the pdf datasheets for those products. Keep in mind, we regularly update our online datasheets to reflect the ...

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Thank you for contacting us regarding the use of ourMitoProfile® Pyruvate dehydrogenase (PDH) WB Antibody Cocktail in rat tissues.

I have gathered the following information regarding each antibodies reactivty with rat below:

...

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Vielen Dank für die Zusammenfassung in Englisch. Ich habe nun eine Antwort von unseren Mitochondrien -Spezialisten aus dem Labor erhalten.

Ich habe die Antwort nicht übersetzt um Übersetzungsfehler zu vermeiden:

"...

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Thank you for your inquiry.

I am happy to confirm the molecular weight of the following proteins:

O (complex 5) : 22kD

E1beta : 39.4kD

E1alpha : 43.3kD

E3bp : 54kD

E2 : 69kD

I also a...

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Thank you for your enquiry. It has taken some time to confirm all the details for you, and I appreciate your patience.

I am sorry to confirm that MS602 has been discontinued and we do not have anymore in stock.

Although that produ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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