Anti-Pyruvate Kinase antibody (HRP) - Azide free (ab34554)


  • Product nameAnti-Pyruvate Kinase antibody (HRP) - Azide free
    See all Pyruvate Kinase primary antibodies
  • Description
    Goat polyclonal to Pyruvate Kinase - Azide free (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: WB, ELISA, Electron Microscopy, Dot blotmore details
  • Species reactivity
    Reacts with: Rabbit
  • Immunogen

    Full length native protein (purified): Pyruvate Kinase from Rabbit muscle



Our Abpromise guarantee covers the use of ab34554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
Dot blot
  • Application notesDot: Use at an assay dependent dilution.
    Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/15000 - 1/60000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 58 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancePyruvate kinase is also known as ATP:pyruvate phosphotransferase. There are 4 isoforms of piruvate kinase in mammals: L, R, M1 and M2. L type is the major isoform in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. Defects in PKLR (pyruvate kinase L and R) are the cause of pyruvate kinase hyperactivity; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP. Also defects in PKLR are a cause of chronic nonspherocytic hemolytic anemia (CNSHA); also called hereditary nonspherocytic hemolytic anemia.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • Pyruvate kinase 3 antibody
      • CTHBP antibody
      • Cytosolic thyroid hormone-binding protein antibody
      • epididymis secretory protein Li 30 antibody
      • HEL-S-30 antibody
      • OIP3 antibody
      • OPA interacting protein 3 antibody
      • p58 antibody
      • PK, muscle type antibody
      • PK1 antibody
      • PK2 antibody
      • PKL antibody
      • PKLR antibody
      • PKM 2 antibody
      • PKM antibody
      • PKM2 antibody
      • PYKM antibody
      • Pyruvate kinase 1 antibody
      • Pyruvate kinase 2/3 antibody
      • Pyruvate kinase isozyme R/L antibody
      • pyruvate kinase isozymes L/R antibody
      • Pyruvate kinase isozymes M1/M2 antibody
      • pyruvate kinase isozymes R/L antibody
      • Pyruvate kinase liver and blood cell antibody
      • Pyruvate kinase liver and RBC antibody
      • Pyruvate kinase muscle antibody
      • Pyruvate kinase muscle isozyme antibody
      • pyruvate kinase PKM antibody
      • pyruvate kinase type L antibody
      • Pyruvate kinase, liver and red blood cell antibody
      • Pyruvate kinase, liver type antibody
      • Pyruvate kinase, muscle, 2 antibody
      • Pyruvate kinase, red cell type antibody
      • R type/L type pyruvate kinase antibody
      • Red cell/liver pyruvate kinase antibody
      • RPK antibody
      • TCB antibody
      • THBP1 antibody
      • Thyroid hormone binding protein cytosolic antibody
      • thyroid hormone-binding protein 1 antibody
      • tumor M2-PK antibody
      see all

    References for Anti-Pyruvate Kinase antibody (HRP) - Azide free (ab34554)

    ab34554 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 100 cells
    Gel Running Conditions Reduced Denaturing (8%)
    Sample Mouse Cell lysate - whole cell (Bone marrow-derived macrophages)
    Specification Bone marrow-derived macrophages
    Treatment poly I:C 1 ug/ml for the indicated times (0, 6, 24 and 48 hours)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Submitted Feb 04 2015

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    Please see below for the anwers to your questions:

    ab34554: This antibody has only been tested with rabbit samples so far. We do not know how well it might work with human samples, but the homology is ...

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