The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. Complement mediated cytotoxicity and the Modulation of Qa2 expression from peripheral T lymphocytes.
RelevanceQa antigens are 'non classical' MHC class I molecules of mice. Qa2 is present on restricted functional lymphocyte subpopulations. The protein product of the preimplantation embryo development (Ped) gene, Qa2 influences the rate of embryonic cleavage during the preimplantation stages of development.
Flow Cytometry analysis of C57BL/6 mouse splenocytes labeling Qa-2 with ab25627 at 0.1 μg/106 cells. A Rat Anti-Mouse CD3ε-FITC was used as the secondary antibody.
References for Anti-Qa 2 antibody [695H1-9-9] (Phycoerythrin) (ab25627)
This product has been referenced in:
Laan M et al. Autoimmune regulator deficiency results in decreased expression of CCR4 and CCR7 ligands and in delayed migration of CD4+ thymocytes. J Immunol183:7682-91 (2009).
Read more (PubMed: 19923453) »
Sharabi Y & Sachs DH In vivo effects of monoclonal antibodies to distinct epitopes of Qa-2 antigens. J Exp Med171:211-9 (1990).
Read more (PubMed: 1688608) »