Overview

  • Product nameAnti-QARS antibody
    See all QARS primary antibodies
  • Description
    Mouse polyclonal to QARS
  • Tested applicationsSuitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human QARS protein (NP_005042.1, 1 a.a. ~ 775 a.a).

  • Positive control
    • Human liver tissue lysate. QARS transfected 293T cell lysate. HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 88 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.

Target

  • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • GlnRS antibody
    • Glutamine tRNA ligase antibody
    • Glutamine tRNA synthetase antibody
    • Glutamine--tRNA ligase antibody
    • Glutaminyl tRNA ligase antibody
    • Glutaminyl tRNA synthetase antibody
    • Glutaminyl-tRNA synthetase antibody
    • PRO2195 antibody
    • QARS antibody
    • SYQ_HUMAN antibody
    see all

Anti-QARS antibody images

  • Anti-QARS antibody (ab72957) at 1 µg/ml + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • All lanes : Anti-QARS antibody (ab72957) at 1 µg/ml

    Lane 1 : QARS transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • ab72957, at 10µg/ml, staining QARS in HeLa cells by immunofluorescence.
  • Cells were lysed hypotonically in 10 mM HEPES pH 7.6, 10 mM KOAc, 1.5 mM Mg(OAc)2, 0.5 mM DTT followed by douncing, followed by salt adjustment to 130 mM KOAc and addition of 1% NP40 for immunoprecipitation.
    Lane 1: input
    Lane 2: supernatant, control antibody
    Lane 3: supernatant, ab72957 at a 1/50 dilution.
    Lane 4: beads. control antibody
    Lane 5: beads, ab72957 at a 1/50 dilution.

    Whole cell lysate prepared from HeLa cells was loaded at 25000000 cells. The immunoprecipitation step was performed using Protein G. For WB ab72957 used at a 1/1000 dilution.

    See Abreview

References for Anti-QARS antibody (ab72957)

ab72957 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa)
Total protein in input 2.5e+007 cells
Specification HeLa
Immuno-precipitation step Protein G
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Abcam user community

Verified customer

Submitted Jul 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cell)
Loading amount 10 µg
Specification HeLa cell
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Feb 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (RAJI B-Cells)
Loading amount 20 µg
Specification RAJI B-Cells
Gel Running Conditions Reduced Denaturing (12% Tris-HEPES-SDS)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Aug 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"