Anti-QDPR antibody [EP12595] (ab186411)

Overview

  • Product name
    Anti-QDPR antibody [EP12595]
    See all QDPR primary antibodies
  • Description
    Rabbit monoclonal [EP12595] to QDPR
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human QDPR aa 50-150. The exact sequence is proprietary.
    Database link: P09417

  • Positive control
    • Mouse brain, mouse heart, mouse kidney, mouse spleen, C6 PC-12, Human fetal liver, K562 lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab186411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.

Target

  • Function
    The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases.
  • Involvement in disease
    Defects in QDPR are the cause of BH4-deficient hyperphenylalaninemia type C (HPABH4C) [MIM:261630]; also called dihydropteridine reductase deficiency (DHPR deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to DHPR deficiency or quinoid dihydropteridine reductase deficiency (QDPR deficiency). HPABH4C is a rare autosomal recessive disorder characterized by hyperphenylalaninemia and severe neurologic symptoms (malignant hyperphenylalaninemia) including axial hypotonia and truncal hypertonia, abnormal thermogenesis, and microcephaly. These signs are attributable to depletion of the neurotransmitters dopamine and serotonin, whose syntheses are controlled by tryptophan and tyrosine hydroxylases that use BH-4 as cofactor. These patients do not respond to phenylalanine-restricted diet. HPABH4C is lethal if untreated.
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6,7 dihydropteridine reductase antibody
    • DHPR antibody
    • DHPR_HUMAN antibody
    • Dihydropteridine reductase antibody
    • HDHPR antibody
    • HPR antibody
    • PKU2 antibody
    • Qdpr antibody
    • Quinoid dihydropteridine reductase antibody
    • SDR33C1 antibody
    • Short chain dehydrogenase/reductase family 33C, member 1 antibody
    see all

Images

  • All lanes : Anti-QDPR antibody [EP12595] (ab186411) at 1/1000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse heart
    Lane 3 : Mouse kidney
    Lane 4 : Mouse spleen
    Lane 5 : C6 cell lysate
    Lane 6 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 26 kDa
  • All lanes : Anti-QDPR antibody [EP12595] (ab186411) at 1/5000 dilution

    Lane 1 : Human fetal liver
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 26 kDa

References

ab186411 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Olfactory epithelium)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Gly Gel)
Loading amount
15 µg
Specification
Olfactory epithelium
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Jun 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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