Overview

  • Product name
  • Description
    Rabbit polyclonal to QKI
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Opossum, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human QKI (C terminal). Synthetic peptide from the C terminus of isoform 5.
    Database link: AAH19917.1

  • Positive control
    • Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab103563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

Target

  • Function
    RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
  • Tissue specificity
    Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
  • Sequence similarities
    Contains 1 KH domain.
  • Domain
    The KH domain and the Qua2 region are involved in RNA binding.
  • Post-translational
    modifications
    Methylated by PRMT1.
    Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586I0923 antibody
    • HKQ antibody
    • Homolog of mouse quaking QKI KH domain RNA binding protein antibody
    • Hqk antibody
    • HQK1 antibody
    • HqkI antibody
    • OTTHUMP00000017581 antibody
    • OTTHUMP00000017582 antibody
    • OTTHUMP00000017583 antibody
    • Protein quaking antibody
    • QK antibody
    • QK1 antibody
    • QK3 antibody
    • QKI antibody
    • QKI_HUMAN antibody
    • QKI1 antibody
    • Quaking homolog antibody
    • Quaking homolog KH domain RNA binding antibody
    • Quaking homolog KH domain RNA binding mouse antibody
    • Quaking isoform 1 antibody
    • Quaking protein antibody
    • RNA binding protein HQK antibody
    see all

Images

  • ab103563, at 1/250 dilution, staining QKI in formalin-fixed, paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry using DAB staining.

References

ab103563 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton X-100
Specification
brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Karen Yee

Verified customer

Submitted Mar 14 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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