Overview

  • Product name
  • Description
    Goat polyclonal to QKI
  • Specificity
    ab77176 is expected to recognise all reported isoforms of QKI.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Human, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human QKI aa 6-17 (N terminal). NP_006766.1; NP_996735.1; NP_996736.1; NP_996737.1
    Sequence:

    ETKEKPKPTPDY-C

  • Positive control
    • Mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 38 kDa.
ELISA 1/64000.

Peptide ELISA

Target

  • Function
    RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
  • Tissue specificity
    Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
  • Sequence similarities
    Contains 1 KH domain.
  • Domain
    The KH domain and the Qua2 region are involved in RNA binding.
  • Post-translational
    modifications
    Methylated by PRMT1.
    Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp586I0923 antibody
    • HKQ antibody
    • Homolog of mouse quaking QKI KH domain RNA binding protein antibody
    • Hqk antibody
    • HQK1 antibody
    • HqkI antibody
    • OTTHUMP00000017581 antibody
    • OTTHUMP00000017582 antibody
    • OTTHUMP00000017583 antibody
    • Protein quaking antibody
    • QK antibody
    • QK1 antibody
    • QK3 antibody
    • QKI antibody
    • QKI_HUMAN antibody
    • QKI1 antibody
    • Quaking homolog antibody
    • Quaking homolog KH domain RNA binding antibody
    • Quaking homolog KH domain RNA binding mouse antibody
    • Quaking isoform 1 antibody
    • Quaking protein antibody
    • RNA binding protein HQK antibody
    see all

Anti-QKI antibody images

  • Anti-QKI antibody (ab77176) at 0.3 µg/ml + mouse brain lysate in RIPA buffer at 35 µg

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-QKI antibody (ab77176)

ab77176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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