Recombinant
RabMAb

Anti-QKI antibody [EPR7306] (ab126742)

Overview

  • Product name
    Anti-QKI antibody [EPR7306]
    See all QKI primary antibodies
  • Description
    Rabbit monoclonal [EPR7306] to QKI
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human QKI aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96PU8

  • Positive control
    • Neuro 2a, HeLa and K562 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab126742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      RNA-binding protein that plays a central role in myelinization (PubMed:16641098). Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability (PubMed:23630077). In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia (PubMed:16641098). Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
    • Tissue specificity
      Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
    • Sequence similarities
      Contains 1 KH domain.
    • Domain
      The KH domain and the Qua2 region are involved in RNA binding.
    • Post-translational
      modifications
      Methylated by PRMT1.
      Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA.
    • Cellular localization
      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp586I0923 antibody
      • HKQ antibody
      • Homolog of mouse quaking QKI KH domain RNA binding protein antibody
      • Hqk antibody
      • HQK1 antibody
      • HqkI antibody
      • OTTHUMP00000017581 antibody
      • OTTHUMP00000017582 antibody
      • OTTHUMP00000017583 antibody
      • Protein quaking antibody
      • QK antibody
      • QK1 antibody
      • QK3 antibody
      • QKI antibody
      • QKI_HUMAN antibody
      • QKI1 antibody
      • Quaking homolog antibody
      • Quaking homolog KH domain RNA binding antibody
      • Quaking homolog KH domain RNA binding mouse antibody
      • Quaking isoform 1 antibody
      • Quaking protein antibody
      • RNA binding protein HQK antibody
      see all

    Images

    • All lanes : Anti-QKI antibody [EPR7306] (ab126742) at 1/1000 dilution

      Lane 1 : Neuro 2a cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 37 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Zou Y  et al. Characteristic expression and significance of CCL19 in different tissue types in chronic rhinosinusitis. Exp Ther Med 11:140-146 (2016). ICC/IF . Read more (PubMed: 26889230) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Recombinant protein (COS-7 monkey cells transfected with mouse QKI)
    Gel Running Conditions
    Reduced Denaturing (18%)
    Loading amount
    200000 cells
    Specification
    COS-7 monkey cells transfected with mouse QKI
    Blocking step
    Odyssey blocking buffer (927-50000) 1:1 in PBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted May 20 2015

    Thank you for contacting us.Jeremy is out of the office today, but I’d be happy to help with your inquiry. My name is Keith.

    In regards to the immunogen sequence which you have requested. Ab78130 is specific for QK1 isoform 1, known ...

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    Thanks for your enquiry.

    I have passed your enquiry regarding the immunogen sequence for ab78518 on to the lab. I will forward you any information I receive.

    The concentration of these 3 antibodies has not been determined. ab78518 a...

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