Validated using a knockout cell line

Anti-QPRT antibody [EPR11941(B)] (ab171944)


  • Product name
    Anti-QPRT antibody [EPR11941(B)]
  • Description
    Rabbit monoclonal [EPR11941(B)] to QPRT
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human QPRT aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15274

  • Positive control
    • Human fetal kidney, Human fetal liver and Human fetal brain lysate, HepG2 and HeLa cell line lysates, Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab171944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC/IF,IHC or IP.
  • Target

    • Function
      Involved in the catabolism of quinolinic acid (QA).
    • Pathway
      Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.
    • Sequence similarities
      Belongs to the nadC/modD family.
    • Information by UniProt
    • Database links
    • Alternative names
      • NADC_HUMAN antibody
      • Nicotinate nucleotide pyrophosphorylase (carboxylating) antibody
      • Nicotinate-nucleotide pyrophosphorylase [carboxylating] antibody
      • QAPRTase antibody
      • QPRT antibody
      • QPRTase antibody
      • Quinolinate phosphoribosyltransferase [decarboxylating] antibody
      • Quinolinate phosphoribosyltransferase antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: QPRT knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: Human fetal brain cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution

      Lane 1 : Human fetal kidney tissue lysate
      Lane 2 : Human fetal liver tissue lysate
      Lane 3 : HepG2 cell line lysate
      Lane 4 : HeLa cell line lysate
      Lane 5 : Jurkat cell line lysate
      Lane 6 : Human fetal heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 31 kDa

    • Flow cytometric analysis of permeabilized Jurkat cells labeling QPRT with ab171944 at 1/10 (red) or a rabbit IgG (negative) (green).


    ab171944 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171944.
    Please use the links above to contact us or submit feedback about this product.


    Sign up