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Abcam's Quick Cell Proliferation Assay Kit II provides by far the easiest and most sensitive means for quantifying cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, just add-and-read, requiring no washing, no harvesting, and no solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.
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|Components||500 tests||2500 tests|
|Electro Coupling Solution (ECS)||1 x 5ml||1 x 25ml|
|Stop Solution||1 x 5ml||1 x 25ml|
|WST Reagent (lyophilized)||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab65475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Mitochondrial dehydrogenases checked in various cell concentrations at different incubation times (2h WST for each condition)
HL60 cell proliferation measured after 24h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
HL60 cell proliferation measured after 48h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
HL60 cell proliferation measured after 72h incubation (37°C/5%CO2) and 2h WST at various camptothecin concentrations
ab65475 has not yet been referenced specifically in any publications.