Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Trafficking >> Organelle Proteins
Overview
Product name
Anti-RAB14 antibody
See all RAB14 products (3) ...
Description
Rabbit polyclonal to RAB14
Tested applications
ICC/IF, ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Pig, Zebrafish
Immunogen
Synthetic peptide derived from a region between amino acids 165-214 of human RAB14. (Note: the exact amino acid sequence is proprietary).
Positive control
Raji cell lysate, Human kidney tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified by peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
ELISA
ELISA: 1/12500ELISA: 1/12500
IHC-P: Use a concentration of 4-8 µg/ml
WB: Use a concentration of 0.5 µg/mlPredicted molecular weight: 24 kDa.
Target
Function
May be involved in vesicular trafficking and neurotransmitter release.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP61106
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- F protein binding protein 1 antibody
- bA165P4.3 antibody
- F protein binding protein 1 antibody
see all
Database links
- Entrez Gene: 51552 Human
- Entrez Gene: 68365 Mouse
- Entrez Gene: 595112 Pig
- Entrez Gene: 94197 Rat
- Entrez Gene: 373104 Zebrafish
- Omim: 612673 Human
- SwissProt: P61106 Human
- SwissProt: Q50HW9 Mouse
see all
Anti-RAB14 antibody images:
Western blot - RAB14 antibody (ab28639)
Anti-RAB14 antibody (ab28639) at 0.5 µg/ml + Raji cell lysate 10ug.
Secondary
HRP conjugated anti-Rabbit IgG
Predicted band size : 24 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - RAB14 antibody (ab28639)
Human kidney tissue stained with ab28639 at X400 magnigication. The arrows point to epithelial cells of the renal tubule [positive label].
Immunocytochemistry/ Immunofluorescence-RAB14 antibody(ab28639)
ICC/IF image of ab28639 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28639, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-RAB14 antibody (ab28639)
This product has been referenced in:
- Mukhopadhyay Aet al. Proteomic analysis of endocytic vesicles: Rab1a regulates motility of early endocytic vesicles. J Cell Sci 124:765-75 (2011).Read more (PubMed: 21303926) »
- Ishikura S & Klip A Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation. Am J Physiol Cell Physiol 295:C1016-25 (2008).Read more (PubMed: 18701652) »
See all 2 publications for this product
Publishing research using ab28639? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"