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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-Rab11 antibody
See all Rab11 products (2) ...
Description
Rabbit polyclonal to Rab11
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Dog, Human, Non Human Primates
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: M(1)GTRDDEYDYLFKVVLIC(17) Immunizing peptide corresponds to amino acid residues 1-17 from human Rab 11. This sequence is completely conserved in human, mouse and rat and 94% conserved in C. elegans and amphibians.
MGTRDDEYDY LFKVVLIC
Positive control
HeLa, PC3 or CV1 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3612 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 24 kDa).(By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.)
Target
Relevance
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There are over 40 Rab proteins in mammals. Rab11a and Rab11b are known markers for protein trafficking, sorting, and recycling in the endosomal pathway. The Rab11 proteins are enriched in recycling endosomes and the trans-Golgi network, where they regulate membrane recycling back to the plasma membrane. Rab11a is ubiquitously expressed, while Rab11b is expressed mainly in the heart and brain.
Cellular localization
Cell Membrane
Alternative names
- GTP binding protein YPT3 antibody
- MGC1490 antibody
- Rab 11 antibody
see all
Database links
- Entrez Gene: 9230 Human
- Entrez Gene: 19326 Mouse
- Entrez Gene: 79434 Rat
- Omim: 604198 Human
- SwissProt: Q15907 Human
- SwissProt: 387587 Mouse
- SwissProt: P46638 Mouse
- SwissProt: O35509 Rat
Anti-Rab11 antibody images:
Immunocytochemistry/ Immunofluorescence - Rab11 antibody (ab3612)
ab3612 staining HEK 293 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.012% saponin prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 37°C. A biotinylated goat anti-rabbit antibody, diluted 1/200, was used as the secondary.
This image depicts ab3612 recognizing endogenous Rab11 (red). Rab11 was used as a recycling endosome marker.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - Anti-Rab11 antibody (ab3612)
ab3612 staining Rab11 in canine kidney cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.5% saponin, then incubated with ab3612 at a 1/200 dilution for 1.5 hours at 20°C. The secondary used was an Alexa-Fluor 568 conjugated donkey anti-rabbit polyclonal, used at a 1/350 dilution.
Image courtesy of an anonymous Abreview.
References for Anti-Rab11 antibody (ab3612)
This product has been referenced in:
- Svendsen Set al. Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter. BMC Cell Biol 9:17 (2008). ICC/IF; Human.Read more (PubMed: 18384686) »
- Li Yet al. The C-terminus of megalin regulates gene expression in opossum kidney proximal tubule (OKP) cells. Am J Physiol Cell Physiol : (2008).Read more (PubMed: 18495814) »
See all 7 publications for this product
Publishing research using ab3612? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"