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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-RAB13 antibody
See all RAB13 products (3) ...
Description
Chicken polyclonal to RAB13
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Immunogen corresponds to full length protein (Human)
Positive control
E coli derived fusion protein.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Western blot - RAB13 antibody (ab37654)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000Detects a band of approximately 75 kDa (predicted molecular weight: 23 kDa).
Target
Function
Could participate in polarized transport, in the assembly and/or the activity of tight junctions.
Tissue specificity
Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell junction > tight junction. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasmic tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions.
Target information above from: UniProt accessionP51153
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell growth-inhibiting gene 4 protein antibody
- GIG4 antibody
- Growth inhibiting gene 4 protein antibody
see all
Database links
- Entrez Gene: 5872 Human
- Entrez Gene: 68328 Mouse
- Entrez Gene: 81756 Rat
- Omim: 602672 Human
- SwissProt: P51153 Human
- SwissProt: Q5U0A6 Human
- SwissProt: Q9DD03 Mouse
- SwissProt: P35286 Rat
see all
Anti-RAB13 antibody images:
Western blot - RAB13 antibody (ab37654)
Anti-RAB13 antibody (ab37654) at 1/2000 dilution + E coli derived fusion protein at 2.5 µg
Secondary
Goat anti IgY HRP at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
The arrow next to the image points to the RAB13 fusion protien.
The use of a fusion protien may account for the difference observed in molecular weight compared to the native protien.
References for Anti-RAB13 antibody (ab37654)
This product has been referenced in:
- Sun Yet al. Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells. Proc Natl Acad Sci U S A 107:19909-14 (2010). WB; Rat.Read more (PubMed: 21041651) »
See 1 publication for this product
Publishing research using ab37654? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"