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Product name
Anti-RAB14 antibody
See all RAB14 products (3) ...
Description
Rabbit polyclonal to RAB14
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Pig, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human RAB14.
(Peptide available as ab418 04.)
Positive control
This antibody gave a positive signal in the following lysates: Brain (Human), Brain (Mouse), Brain (Rat) and Raji (data not shown).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Golgi Proteins
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 24 kDa).
Target
Function
May be involved in vesicular trafficking and neurotransmitter release.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP61106
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- F protein binding protein 1 antibody
- bA165P4.3 antibody
- FBP antibody
see all
Database links
- Entrez Gene: 417119 Chicken
- Entrez Gene: 51552 Human
- Entrez Gene: 68365 Mouse
- Entrez Gene: 595112 Pig
- Entrez Gene: 94197 Rat
- Omim: 612673 Human
- SwissProt: P61106 Human
- SwissProt: Q50HW9 Mouse
see all
Anti-RAB14 antibody images:
Western blot - RAB14 antibody (ab40938)
All lanes : Anti-RAB14 antibody (ab40938) at 1 µg/ml
Lane 1 :
Lane 2 : Brain (Mouse) Mouse Tissue Lysate
Lane 3 : Brain (Rat) Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 24 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
The RAB14 protein has several Ser/Thr residues which may be phosphorylated (SwissProt data). This could cause the doublet observed at the expected molecular weight.
Immunocytochemistry/ Immunofluorescence - RAB14 antibody (ab40938)
ab40938 staining RAB14 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. HeLa cells were infected with C. trachomatis serovar L2 (MOI 5) for 10 hours. The cells were fixed in paraformaldehyde, permeabilised with saponin and then blocked using 0.2% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to FITC (green) used at a 1/200 dilution. Microtubules were evinced by a mouse monoclonal anti-ß-tubulin (1/200) followed by donkey anti-mouse Cy5-labeled antibodies (1/700) (red). Endogenous Rab14 was recruited to the chlamydial inclusion (blue asterisk). Bacteria were labeled with Hoechst 0.1 mg/ml (blue). ab40938 was useful to detect for the first time the recruitment of an endogenous RAB to the chlamydial inclusion.
Image courtesy of Dr María Damiani by Abreview.
References for Anti-RAB14 antibody (ab40938)
This product has been referenced in:
- Comellas AP et al. Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt. J Cell Sci 123:1343-51 (2010). WB; Human.Read more (PubMed: 20332111) »
- Capmany A & Damiani MT Chlamydia trachomatis intercepts Golgi-derived sphingolipids through a Rab14-mediated transport required for bacterial development and replication. PLoS One 5:e14084 (2010). ICC/IF; Human.Read more (PubMed: 21124879) »
See all 2 publications for this product
Publishing research using ab40938? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"