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Products:Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Overview
Product name
Anti-Rab9 antibody
See all Rab9 products (6) ...
Description
Rabbit polyclonal to Rab9
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab9.
(Peptide available as ab45894.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Spleen (Mouse) and Spleen (Rat).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Endosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Target
Function
Involved in the transport of proteins between the endosomes and the trans Golgi network.
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane.
Target information above from: UniProt accessionP51151
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2410064E05Rik antibody
- AI195561 antibody
- DmRab9 antibody
see all
Database links
- Entrez Gene: 9367 Human
- Entrez Gene: 56382 Mouse
- Entrez Gene: 84589 Rat
- Omim: 300284 Human
- SwissProt: P51151 Human
- SwissProt: Q9R0M6 Mouse
- SwissProt: Q99P75 Rat
- Unigene: 495704 Human
see all
Anti-Rab9 antibody images:
Western blot - Rab9 antibody (ab45864)
All lanes : Anti-Rab9 antibody (ab45864) at 1 µg/ml
Lane 1 : Spleen (Mouse) Tissue Lysate (ab29293)
Lane 2 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 23 kDa
Observed band size : 23 kDa
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody (ab45864)
IHC image of ab45864 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45864, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Rab9 antibody (ab45864)
ab45864 has not yet been referenced specifically in any publications.
Publishing research using ab45864? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"